Delayed repair of the facial nerve and its negative impacts on nerve and muscle

被引：0

作者：

Bueno, Cleuber Rodrigo de Souza ^{[1
,2
,3
]}

Buchaim, Daniela Vieira ^{[3
,4
,5
]}

Barraviera, Benedito ^{[6
,7
]}

Ferreira Jr, Rui Seabra ^{[8
]}

Santos, Paulo Sergio da Silva ^{[8
]}

Reis, Carlos Henrique Bertoni ^{[1
,4
,9
]}

Cini, Marcelo Augusto ^{[1
,10
]}

Kuga, Milton Carlos ^{[1
,11
]}

Rosa Junior, Geraldo Marco ^{[12
]}

Buchaim, Rogerio Leone ^{[1
,5
]}

机构：

[1] Univ Sao Paulo, Bauru Sch Dent FOB, Dept Biol Sci, Bauru, SP, Brazil

[2] Univ Ctr Adamantina UNIFAI, Dent Sch, Adamantina, SP, Brazil

[3] Univ Ctr Adamantina UNIFAI, Med Sch, Adamantina, SP, Brazil

[4] Univ Marilia UNIMAR, Grad Program Struct & Funct Interact Rehabil, Marilia, SP, Brazil

[5] Univ Sao Paulo, Fac Vet Med & Anim Sci FMVZ, Grad Program Anat Domest & Wild Anim, Sao Paulo, SP, Brazil

[6] Sao Paulo State Univ UNESP, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP, Brazil

[7] Sao Paulo State Univ UNESP, Botucatu Med Sch FMB, Grad Program Trop Dis, Botucatu, SP, Brazil

[8] Univ Sao Paulo, Bauru Sch Dent FOB, Dept Surg Stomatol Pathol & Radiol, Bauru, SP, Brazil

[9] Univ Marilia UNIMAR, UNIMAR Beneficent Hosp HBU, Marilia, SP, Brazil

[10] Univ West Paulista UNOESTE, Med Sch, Guaruja, SP, Brazil

[11] Sao Paulo State Univ UNESP, Sch Dent, Dept Restorat Dent, Araraquara, SP, Brazil

[12] Fac Midwest Paulista FACOP, Dent Sch, Piratininga, SP, Brazil

来源：

JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES | 2024年 / 30卷

基金：

巴西圣保罗研究基金会;

关键词：

Facial nerve; Nerve regeneration; Photobiomodulation; Fibrin sealant; Biopolymers; LEVEL LASER THERAPY; FIBRIN SEALANT; INJURY; PHOTOBIOMODULATION; REGENERATION; STIMULATION; MANAGEMENT; RECOVERY; LLLT; GLUE;

D O I：

10.1590/1678-9199-JVATITD-2023-0093

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Background: In this experimental protocol, we evaluated the immediate and delayed repair of the buccal branch of the facial nerve (BBFN) with heterologous fibrin biopolymer (HFB) as a coaptation medium and the use of photobiomodulation (PBM), performing functional and histomorphometric analysis of the BBFN and perioral muscles. Methods: Twenty-eight rats were divided into eight groups using the BBFN bilaterally (the left nerve was used for PBM), namely: G1 - control group, right BBFN (without injury); G2 - control group, left BBFN (without injury + PBM); G3 - Denervated right BBFN (neurotmesis); G4 - Denervated left BBFN (neurotmesis + PBM); G5 - Immediate repair of right BBFN (neurotmesis + HFB); G6 - Immediate repair of left BBFN (neurotmesis + HFB + PBM); G7 - Delayed repair of right BBFN (neurotmesis + HFB); G8 - Delayed repair of left BBFN (neurotmesis + HFB + PBM). Delayed repair occurred after two weeks of denervation. All animals were sacrificed after six weeks postoperatively. Results: In the parameters of the BBFN, we observed inferior results in the groups with delayed repair, in relation to the groups with immediate repair, with a significant difference (p < 0.05) in the diameter of the nerve fiber, the axon, and the thickness of the myelin sheath of the group with immediate repair with PBM compared to the other experimental groups. In measuring the muscle fiber area, groups G7 (826.4 +/- 69.90) and G8 (836.7 +/- 96.44) were similar to G5 (882.8 +/- 70.51). In the functional analysis, the G7 (4.10 +/- 0.07) and G8 (4.12 +/- 0.08) groups presented normal parameters. Conclusion: We demonstrated that delayed repair of BBFN is possible with HFB, but with worse results compared to immediate repair, and that PBM has a positive influence on nerve regeneration results in immediate repair.

引用

页数：14