Seroprevalence of Toxoplasma gondii among HIV Positive Patients under Surveillance in Greek Infectious Disease Units: A Screening Study with Comparative Evaluation of Serological Methods

被引:1
作者
Voyiatzaki, Chrysa [1 ]
Chormizi, Apollon Dareios Zare [1 ]
Tsoumani, Maria E. [1 ]
Efstathiou, Antonia [1 ,2 ]
Konstantinidis, Konstantinos [3 ]
Chaniotis, Dimitrios [1 ]
Chrysos, Georgios [4 ]
Argyraki, Aikaterini [5 ]
Papastamopoulos, Vasileios [6 ]
Kotsianopoulou, Marika [7 ]
机构
[1] Univ West Attica, Dept Biomed Sci, Div Med Labs Sci, Athens 12243, Greece
[2] Hellenic Pasteur Inst, Dept Microbiol, Immunol Infect Grp, Athens 11521, Greece
[3] Democritus Univ Thrace, Dept Med, Lab Biol, Dragana 68100, Alexandroupolis, Greece
[4] Tzaneio Gen Hosp Piraeus, Dept Internal Med 2, Athens 18536, Greece
[5] Sotiria Thorac Dis Gen Hosp, Dept Internal Med, Athens 11527, Greece
[6] Evaggelismos Gen Hosp, Dept Internal Med 5, Infect Dis Unit, Athens 10676, Greece
[7] Univ West Attica, Sch Publ Hlth, Dept Publ Hlth Policy, Athens 11521, Greece
关键词
opportunistic infection; infectious disease; toxoplasmosis; Europe/Greece; comparative serology; ELISA; IFAT; WB; PEOPLE;
D O I
10.3390/pathogens13050375
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study aims to screen for IgG antibodies against Toxoplasma gondii (T. gondii) in the sera of 155 newly diagnosed Human Immunodeficiency Virus (HIV) positive patients under surveillance in Greek Infectious Disease Units. Additionally, risk factors based on patient demographics were examined, and a comparative evaluation of commercially available serological methods was conducted. Three methods were employed to detect IgG antibodies against T. gondii: Enzyme-Linked Immunosorbent Assay (ELISA), Indirect Immunofluorescence Antibody Test (IFAT), and Western Blot (WB), which was used as a reference here. Forty-nine sera samples were true-positive for IgG antibodies against T. gondii, resulting in a 31.61% positivity rate, and the immunoassay test statistical reliability analysis resulted in higher IFAT accuracy (90.97%) compared to ELISA (76.26%). Furthermore, statistical analysis of demographic and immunological data included in the study placed female and foreign/non-Greek individuals at 2.24 (p = 0.0009) and 2.34 (p = 0.0006) times higher risk of positive T. gondii IgG testing compared to their male and Greek counterparts, respectively. Our findings on positivity rates and comparative serology underscore the importance of early and suitable screening measures for newly diagnosed HIV+ patients to mitigate the life-threatening outcomes that may arise from a potential subsequent T. gondii activation.
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共 18 条
[1]   Hormonal modulation of Toxoplasma gondii infection: Regulation of hormonal receptors and cytokine production in THP-1 cells [J].
de la Luz Galvan-Ramirez, Maria ;
Ramirez De Arellano, Adrian ;
Rocio Rodriguez-Perez, Laura ;
Lopez-Pulido, Edgar, I ;
Francisco Munoz-Valle, Jose ;
Laura Pereira-Suarez, Ana .
EXPERIMENTAL PARASITOLOGY, 2019, 204
[2]   Serology for Toxoplasma in Immunocompromised Patients: Still Useful? [J].
Dupont, Damien ;
Fricker-Hidalgo, Helene ;
Brenier-Pinchart, Marie-Pierre ;
Garnaud, Cecile ;
Wallon, Martine ;
Pelloux, Herve .
TRENDS IN PARASITOLOGY, 2021, 37 (03) :205-213
[3]   LDBio-Toxo II immunoglobulin G Western blot confirmatory test for anti-Toxoplasma antibody detection [J].
Franck, Jacqueline ;
Garin, Yves Jean-Francois ;
Dumon, Henri .
JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (07) :2334-2338
[4]   Prevalence of ocular toxoplasmosis among people living with HIV: a systematic review and meta-analysis [J].
Kantzanou, Maria ;
Kostares, Evangelos ;
Kostare, Georgia ;
Boufidou, Fotini ;
Tzanai, Antzela ;
Kostares, Michael ;
Tsakris, Athanasios .
FUTURE MICROBIOLOGY, 2024, 19 (06) :525-534
[5]   Incidence, immunological and clinical characteristics of reactivation of latent Toxoplasma gondii infection in HIV-infected patients [J].
Kodym, P. ;
Maly, M. ;
Beran, O. ;
Jilich, D. ;
Rozsypal, H. ;
Machala, L. ;
Holub, M. .
EPIDEMIOLOGY AND INFECTION, 2015, 143 (03) :600-607
[6]   Incidence, presentation and outcome of toxoplasmosis in HIV infected in the combination antiretroviral therapy era [J].
Martin-Iguacel, Raquel ;
Ahlstrom, Magnus Glindvad ;
Touma, Madeleine ;
Engsig, Frederik Neess ;
Staerke, Nina Breinholt ;
Staerkind, Mette ;
Obel, Niels ;
Rasmussen, Line D. .
JOURNAL OF INFECTION, 2017, 75 (03) :263-273
[7]   Toxoplasma gondii infection and its implications within the central nervous system [J].
Matta, Sumit K. ;
Rinkenberger, Nicholas ;
Dunay, Ildiko R. ;
Sibley, L. David .
NATURE REVIEWS MICROBIOLOGY, 2021, 19 (07) :467-480
[8]   Bicentric Evaluation of Six Anti-Toxoplasma Immunoglobulin G (IgG) Automated Immunoassays and Comparison to the Toxo II IgG Western Blot [J].
Maudry, Arnaud ;
Chene, Gautier ;
Chatelain, Remi ;
Patural, Hugues ;
Bellete, Bahrie ;
Tisseur, Bernard ;
Hafid, Jamal ;
Raberin, Helene ;
Beretta, Sophie ;
Sung, Roger Tran Manh ;
Belot, Georges ;
Flori, Pierre .
CLINICAL AND VACCINE IMMUNOLOGY, 2009, 16 (09) :1322-1326
[9]   Seronegative Infection with Toxoplasma gondii in Asymptomatic Human Immunodeficiency Virus Type 1 (HIV-1)-Infected Patients and in Blood Donors [J].
Pawelczyk, Agnieszka ;
Bednarska, Malgorzata ;
Caraballo Cortes, Kamila ;
Glamkowska-Sady, Marianna ;
Kowalska, Justyna ;
Uszynska-Kaluza, Beata ;
Radkowski, Marek ;
Welc-Faleciak, Renata .
JOURNAL OF CLINICAL MEDICINE, 2022, 11 (03)
[10]   Epidemiology of and Diagnostic Strategies for Toxoplasmosis [J].
Robert-Gangneux, Florence ;
Darde, Marie-Laure .
CLINICAL MICROBIOLOGY REVIEWS, 2012, 25 (02) :264-296