Type I arginine methyltransferases play crucial roles in development and pathogenesis of Phytophthora capsici

被引:0
作者
Zhang, Sicong [1 ]
Zhang, Borui [1 ]
Wang, Zhiwen [1 ,2 ]
Zhong, Shan [1 ]
Zheng, Yang [1 ]
Zhang, Qinghua [1 ]
Liu, Xili [1 ,3 ]
机构
[1] China Agr Univ, Coll Plant Protect, Beijing 100193, Peoples R China
[2] Sanya Inst China Agr Univ, Sanya 572025, Peoples R China
[3] Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Shaanxi 712100, Peoples R China
关键词
Phytophthora capsici; Arginine methyltransferases; Biological processes; Oxidative stress; Virulence; GENE-EXPRESSION; NONHISTONE PROTEINS; HISTONE H4; METHYLATION; RNA; PEROXISOMES; REVEALS; BINDING; PHOSPHORYLATION; REGULATOR;
D O I
10.1016/j.ijbiomac.2024.134671
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phytophthora capsici, a pathogenic oomycete, poses a serious threat to global vegetable production. This study investigated the role of protein arginine methylation, a notable post-translational modification, in the epigenetic regulation of P. capsici. We identified and characterized five protein arginine methyltransferases (PRMTs) in P. capsici, with a focus on four putative type I PRMTs exhibiting similar functional domain. Deletion of PcPRMT3, a homolog of PRMT3, significantly affected mycelial growth, asexual spore development, pathogenicity, and stress responses in P. capsici. Transcriptome analyses indicated that absence of PcPRMT3 disrupted multiple biological pathways. The PcPRMT3 deletion mutant displayed heightened susceptibility to oxidative stress, correlated with the downregulation of genes involved in peroxidase and peroxisome activities. Additionally, PcPRMT3 acted as a negative regulator, modulating the transcription levels of specific elicitins, which in turn affects the defense response of host plant against P. capsici. Furthermore, PcPRMT3 was found to affect global arginine methylation levels in P. capsici, implying potential alterations in the functions of its substrate proteins.
引用
收藏
页数:13
相关论文
共 92 条
  • [1] Characterization of the PRMT Gene Family in Rice Reveals Conservation of Arginine Methylation
    Ahmad, Ayaz
    Dong, Yuzhu
    Cao, Xiaofeng
    [J]. PLOS ONE, 2011, 6 (08):
  • [2] MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana
    Asai, Shuta
    Ohta, Kohji
    Yoshioka, Hirofumi
    [J]. PLANT CELL, 2008, 20 (05) : 1390 - 1406
  • [3] Protein Arginine Methylation in Mammals: Who, What, and Why
    Bedford, Mark T.
    Clarke, Steven G.
    [J]. MOLECULAR CELL, 2009, 33 (01) : 1 - 13
  • [4] Arginine methylation: An emerging regulator of protein function
    Bedford, MT
    Richard, S
    [J]. MOLECULAR CELL, 2005, 18 (03) : 263 - 272
  • [5] Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2
    Bikkavilli, Rama Kamesh
    Malbon, Craig C.
    [J]. JOURNAL OF CELL SCIENCE, 2012, 125 (10) : 2446 - 2456
  • [6] Reactive oxygen species and peroxisomes: Struggling for balance
    Bonekamp, Nina A.
    Voelkl, Alfred
    Fahimi, H. Dariush
    Schrader, Michael
    [J]. BIOFACTORS, 2009, 35 (04) : 346 - 355
  • [7] A SEEDLING SCREEN FOR PHYTOPHTHORA ROOT-ROT OF PEPPER, CAPSICUM-ANNUUM
    BOSLAND, PW
    LINDSEY, DL
    [J]. PLANT DISEASE, 1991, 75 (10) : 1048 - 1050
  • [8] Chandra S., 2023, DEV APPL MICROBIOLOG, P77, DOI [10.1016/B978-0-323-99896-3.00001-1, DOI 10.1016/B978-0-323-99896-3.00001-1]
  • [9] A discussion on disease severity index values. Part II: using the disease severity index for null hypothesis testing
    Chiang, K. S.
    Liu, H. I.
    Tsai, J. W.
    Tsai, J. R.
    Bock, C. H.
    [J]. ANNALS OF APPLIED BIOLOGY, 2017, 171 (03) : 490 - 505
  • [10] Nine things to know about elicitins
    Derevnina, Lida
    Dagdas, Yasin F.
    De la Concepcion, Juan Carlos
    Bialas, Aleksandra
    Kellner, Ronny
    Petre, Benjamin
    Domazakis, Emmanouil
    Du, Juan
    Wu, Chih-Hang
    Lin, Xiao
    Aguilera-Galvez, Carolina
    Cruz-Mireles, Neftaly
    Vleeshouwers, Vivianne G. A. A.
    Kamoun, Sophien
    [J]. NEW PHYTOLOGIST, 2016, 212 (04) : 888 - 895