Microfluidic paper-based chemiluminescence sensing platform based on functionalized CaCO3 for time-resolved multiplex detection of avian influenza virus biomarkers

被引：5
作者：
Tian, Yafei [1 ]
Zhang, Yujiao [1 ]
Lu, Xueyun [1 ]
Xiao, Dan [1 ]
Zhou, Cuisong [1 ]
机构：
[1] Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China
来源：
ANALYTICAL BIOCHEMISTRY | 2024年 / 693卷
基金：
中国国家自然科学基金;
关键词：
Chemiluminescence; mu PAD; CHA; Avian influenza virus; SENSITIVE DETECTION; RAPID DETECTION; IMMUNOSENSOR; NANORODS; DEVICE; H5N1;
D O I：
10.1016/j.ab.2024.115583
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Multiplex detection can enhance diagnostic precision and improve diagnostic efficiency, providing important assistance for epidemiological investigation and epidemic prevention. There is a great need for multi-detection sensing platforms to accurately diagnose diseases. Herein, we reported a mu PAD-based chemiluminescence (CL) assay for ultrasensitive multiplex detection of AIV biomarkers, based on three DNAzyme/Lum/PEI/CaCO3. Three time-resolved CL signals were sequentially generated with detection limits of 0.32, 0.34, and 0.29 pM for H1N1, H7N9, and H5N1, respectively, and with excellent selectivity against interfering DNA. The recovery test in human serum displayed satisfactory analysis capabilities for complex biological samples. The mu PAD-based CL assay achieved multiplex detection within 70 s, with a high time resolution of 20 s. The proposed strategy has the advantages of low cost, high sensitivity, good selectivity, and wide time resolution, the mu PAD-based CL assay has shown great potential in the early and accurate diagnosis of diseases.
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页数：8
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