Genomic Differences Associated with Resistance and Virulence in Pseudomonas aeruginosa Isolates from Clinical and Environmental Sites

被引:2
|
作者
Molina, Kelly J. Aroca [1 ]
Gutierrez, Sonia Jakeline [1 ]
Benitez-Campo, Neyla [1 ]
Correa, Adriana [2 ]
机构
[1] Univ Valle, Fac Nat & Exact Sci, Dept Biol, Cali 760042, Colombia
[2] Univ Santiago Cali, Dept Basic Sci, Cali 760035, Colombia
关键词
antibiotic resistance; comparative genome; Pseudomonas aeruginosa; virulence; whole-genome sequencing; ANTIBIOTIC-RESISTANCE; PATTERNS; GENES; IDENTIFICATION; DISSEMINATION; BIOSYNTHESIS; SIDEROPHORES; DIVERSITY; PATHOGEN; STRAIN;
D O I
10.3390/microorganisms12061116
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pseudomonas aeruginosa is a pathogen that causes healthcare-associated infections (HAIs) worldwide. It is unclear whether P. aeruginosa isolated from the natural environment has the same pathogenicity and antimicrobial resistance potential as clinical strains. In this study, virulence- and resistance-associated genes were compared in 14 genomic sequences of clinical and environmental isolates of P. aeruginosa using the VFDB, PATRIC, and CARD databases. All isolates were found to share 62% of virulence genes related to adhesion, motility, secretion systems, and quorum sensing and 72.9% of resistance genes related to efflux pumps and membrane permeability. Our results indicate that both types of isolates possess conserved genetic information associated with virulence and resistance mechanisms regardless of the source. However, none of the environmental isolates were associated with high-risk clones (HRCs). These clones (ST235 and ST111) were found only in clinical isolates, which have an impact on human medical epidemiology due to their ability to spread and persist, indicating a correlation between the clinical environment and increased virulence. The genomic variation and antibiotic susceptibility of environmental isolates of P. aeruginosa suggest potential biotechnological applications if obtained from sources that are under surveillance and investigation to limit the emergence and spread of antibiotic resistant strains
引用
收藏
页数:18
相关论文
共 50 条
  • [1] Antimicrobial susceptibility and virulence genes of clinical and environmental isolates of Pseudomonas aeruginosa
    Liew, Siew Mun
    Rajasekaram, Ganeswrei
    Puthucheary, S. D. Ampalam
    Chua, Kek Heng
    PEERJ, 2019, 7
  • [2] Antimicrobial resistance, virulence factors, and genotypes of Pseudomonas aeruginosa clinical isolates from Gorgan, northern Iran
    Shahri, Fatemeh Nemati
    Izanloo, Ahdieh
    Goharrizi, Mohammad Ali Sheikh Beig
    Jamali, Ailar
    Bagheri, Hanieh
    Hjimohammadi, Afsaneh
    Ardebili, Abdollah
    INTERNATIONAL MICROBIOLOGY, 2022, 25 (04) : 709 - 721
  • [3] Correlation between phenotypic virulence traits and antibiotic resistance in Pseudomonas aeruginosa clinical isolates
    Nassar, Osama
    Desouky, Said E.
    El-Sherbiny, Gamal M.
    Abu-Elghait, Mohammed
    MICROBIAL PATHOGENESIS, 2022, 162
  • [4] Identification of genomic loci associated with genotypic and phenotypic variation among Pseudomonas aeruginosa clinical isolates from pneumonia
    Mesquita, Cristina S.
    Soares-Castro, Pedro
    Faustino, Alberta
    Santos, Hugo M.
    Capelo, Jose L.
    Santos, Pedro
    MICROBIAL PATHOGENESIS, 2019, 136
  • [5] Comparison of virulence between clinical and environmental Pseudomonas aeruginosa isolates
    Vives-Florez, Martha
    Garnica, Diana
    INTERNATIONAL MICROBIOLOGY, 2006, 9 (04) : 247 - 252
  • [6] Antibiotic Resistance and Virulence Determinants of Pseudomonas aeruginosa Isolates Cultured from Hydrocarbon-Contaminated Environmental Samples
    Ozoaduche, Chioma Lilian
    Libisch, Balazs
    Itoro, Daniel
    Idemudia, Iyore Blessing
    Posta, Katalin
    Olasz, Ferenc
    MICROORGANISMS, 2025, 13 (03)
  • [7] Resistance to antibiotics in clinical isolates of Pseudomonas aeruginosa
    Sevillano, E.
    Valderrey, C.
    Canduela, M. J.
    Umaran, A.
    Calvo, F.
    Gallego, L.
    PATHOLOGIE BIOLOGIE, 2006, 54 (8-9): : 493 - 497
  • [8] Comparing antibiotic resistance and virulence profiles of Enterococcus faecium, Klebsiella pneumoniae, and Pseudomonas aeruginosa from environmental and clinical settings
    Denissen, Julia
    Havenga, Benjamin
    Reyneke, Brandon
    Khan, Sehaam
    Khan, Wesaal
    HELIYON, 2024, 10 (09)
  • [9] Comparison of biofilm formation and antibiotic resistance pattern of Pseudomonas aeruginosa in human and environmental isolates
    Gholami, Sayyad
    Tabatabaei, Mohammad
    Sohrabi, Nasrollah
    MICROBIAL PATHOGENESIS, 2017, 109 : 94 - 98
  • [10] Virulence attributes in Brazilian clinical isolates of Pseudomonas aeruginosa
    Silva, Livia V.
    Galdino, Anna Clara M.
    Nunes, Ana Paula F.
    dos Santos, Katia R. N.
    Moreira, Beatriz M.
    Cacci, Luciana C.
    Sodre, Catia L.
    Ziccardi, Mariangela
    Branquinha, Marta H.
    Santos, Andre L. S.
    INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2014, 304 (08) : 990 - 1000