Spatial Cytoskeleton Organization Supports Targeted Intracellular Transport

被引:20
作者
Hafner, Anne E. [1 ,2 ]
Rieger, Heiko [1 ,2 ]
机构
[1] Saarland Univ, Dept Theoret Phys, Saarbrucken, Germany
[2] Saarland Univ, Ctr Biophys, Saarbrucken, Germany
关键词
NARROW ESCAPE PROBLEM; MOLECULAR MOTORS; IMMUNOLOGICAL SYNAPSE; CARGO TRANSPORT; ACTIN CORTEX; MEMBRANE REPAIR; LIVING CELLS; MICROTUBULES; DYNAMICS; MOVEMENT;
D O I
10.1016/j.bpj.2018.01.042
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The efficiency of intracellular cargo transport from specific sources to target locations is strongly dependent upon molecular motor-assisted motion along the cytoskeleton. Radial transport along microtubules and lateral transport along the filaments of the actin cortex underneath the cell membrane are characteristic for cells with a centrosome. The interplay between the specific cytoskeleton organization and the motor performance results in a spatially inhomogeneous intermittent search strategy. To analyze the efficiency of such intracellular search strategies, we formulate a random velocity model with intermittent arrest states. We evaluate efficiency in terms of mean first passage times for three different, frequently encountered intracellular transport tasks: 1) the narrow escape problem, which emerges during cargo transport to a synapse or other specific region of the cell membrane; 2) the reaction problem, which considers the binding time of two particles within the cell; and 3) the reaction-escape problem, which arises when cargo must be released at a synapse only after pairing with another particle. Our results indicate that cells are able to realize efficient search strategies for various intracellular transport tasks economically through a spatial cytoskeleton organization that involves only a narrow actin cortex rather than a cell body filled with randomly oriented actin filaments.
引用
收藏
页码:1420 / 1432
页数:13
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