Green synthesis of ultrafine gold nanoparticles from sweet flag ( Acorus calamus ) for effective anti-leukemic, anti-urolithiatic and in silico docking studies

Gold nanoparticles (AuNPs) are emerging effective biomaterials which play a vital role in the biodiversity research area due to their biocompatibility, and less toxic nature. The AuNPs of various sizes and shapes have vibrant optical features that can be utilized for a variety of imaging and therapeutic applications. Acorus calamus ( A. calamus ) has been used in traditional medical systems as a general tonic for centuries. It is thought to have potential benefits in the treatment of digestive disorders and respiratory problems. In Kerala, newborns were given a nanogram of ( A. calamus ) rhizome paste to increase oral cleanliness and improve their health and immunity. This prompted us to produce AuNPs by A. calamus and to explore their concealed pharmacological properties. In this work, we used a simple sonication method to synthesise blue -coloured AuNPs using sweet flag ( A. calamus ) extract, which acted as a stabilising and reducing agent at ambient temperature. The formation and morphology of the AC-AuNPs was confirmed by XRD, TEM, EDX, FT-IR and UV -vis spectral techniques. Antileukemic and anti-urolithiatic activities of the AC-AuNPs were also investigated. The anti -leukemic results revealed cytomorphological changes like cancer cell membrane lyses and coiling in Human myelogenous leukemia cells (K562) and exhibited IC 50 at a concentration of 41.45 mu g/mL. Furthermore, in vitro DNA binding assay of AC-AuNPs with calf -thymus were conducted. The anti-urolithiatic activity of AC-AuNPs suggests a dosedependent suppression of calcium oxalate method (COM) crystals nucleation and aggregation. These results suggest that AC-AuNPs have potent antileukemic and anti-urolithiatic activities. In silico docking results revealed that eugenol, alpha-asarone and calamenene possess good binding scores with myeloid cell surface antigen (CD33) and estrogen receptors, respectively. In vitro anticancer and DNA binding studies have endorsed the capping nature of AC-AuNPs by these phytoconstituents, which is validated by docking analysis.