DHA dietary intervention caused different hippocampal lipid and protein profile in ApoE-/- and C57BL/6J mice

被引：3

作者：

Liu, Lu ^{[1
,2
]}

Xu, Jingjing ^{[1
,2
]}

Huang, Xiaochen ^{[1
]}

Wang, Ying ^{[3
]}

Ma, Xiaojun ^{[1
,2
]}

Wang, Xixiang ^{[1
,2
]}

Liu, Yu ^{[1
,2
]}

Ren, Xiuwen ^{[1
,2
]}

Li, Jiahao ^{[1
,2
]}

Wang, Yueyong ^{[1
,2
]}

Zhou, Shaobo ^{[4
]}

Yuan, Linhong ^{[1
,2
]}

机构：

[1] Capital Med Univ, Sch Publ Hlth, Beijing, Peoples R China

[2] China British Joint Lab Nutr Prevent & Control Chr, Beijing, Peoples R China

[3] Nanjing Univ, Suzhou Hosp, Affiliated Hosp, Med Sch, Suzhou, Peoples R China

[4] Univ Greenwich, Fac Engn & Sci, Sch Sci, Cent Ave, Chatham ME4 4TB, England

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2024年 / 177卷

基金：

中国国家自然科学基金;

关键词：

ApoE-/-mice; DHA; Dietary intervention proteomics; Lipidomics; CHOLESTEROL EFFLUX; ALZHEIMERS-DISEASE; BRAIN; RECEPTOR; ACID; METABOLISM; EXPRESSION; DISORDERS; TRANSPORT; HDL;

D O I：

10.1016/j.biopha.2024.117088

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background: Changes in protein and lipid levels may occur in the Alzheimer's disease brain, and DHA can have beneficial effects on it. To investigate the impact of DHA dietary intervention on brain protein and lipid profile in ApoE-/mice and C57 mice. Method: Three-month-old ApoE-/mice and C57 mice were randomly divided into two groups respectively, and fed with control diet and DHA-fortified diet for five months. Cortical TC, HDL-C and LDL-C levels and cholesterol metabolism-related protein expression were measured by ELISA or immunohistochemistry methods. Hippocampus were collected for proteomic and lipidomics analysis by LC-MS/MS and differential proteins and lipid metabolites were screened and further analyzed by GO functional annotation and KEGG pathway enrichment analysis. Results: DHA intervention decreased cortical TC level in both C57 and ApoE-/mice (P < 0.05), but caused different change of cortical HDL-C, LDL-C level and LDL-C/HDL-C ratio in C57 and ApoE-/mice (P < 0.05). Discrepant cortical and hippocampal LDLR, ABCG1, Lox1 and SORT1 protein expression was found between C57 and ApoE-/mice (P < 0.05), and DHA treatment caused different changes of these proteins in C57 and ApoE-/mice (P < 0.05). Differential hippocampal proteins and lipids profile were found in C57 and ApoE-/mice before and after DHA treatment, which were mainly involved in vesicular transport and phospholipid metabolic pathways. Conclusion: ApoE genetic defect caused abnormal cholesterol metabolism, and affected protein and lipid profile, as well as discrepant response of hippocampal protein and lipids profile in the brain of mice given DHA fortified diet intervention.

引用

页数：16

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