Phytochemical Constituents, Antioxidant, and Antibacterial Activities of Himalayan Indigofera Dosua Leaf Extract

The methanolic extract of Indigofera dosua leaves was subjected to GC-MS analysis, its antioxidant and antimicrobial potential. Sixteen compounds were identified having resorcinol TMS derivatives, propanoic acid, dimethyl(ethenyl)silyl ester, 4-O-methylmannose, hexadecanoic acid, trimethylsilyl ester, 9,12,15-octadecatriene-1-ol, and phytol as major components. FRAP content was 40.24 +/- 0.54AAE/g, and the IC50 values were 8.25 +/- 0.04 mu g mL-1 and 19.05 +/- 0.15 mu g mL-1 when employing DPPH and ABTS, respectively. Total phenolics (50.2 +/- 0.48 mg GAE/g) and total flavonoid content (5.33 +/- 0.40 mg QE/g) were estimated. Antibacterial activity was observed, showing significant inhibition against Pseudomonas aeruginosa and Proteus mirabilis with MIC values 12.5 and 25 mg mL-1, respectively.