Method: 2D/3D kV imaging and CBCT data using 6 degrees of freedom (6DoF) were compared to evaluate inter and intrafraction motion. Results: Results showed that intrafraction errors were low and interfraction levels were within institutional protocols. Conclusion: Con fidence was given to use low dose 2D/3D kV imaging to con firm daily patient set up errors, and to use pre-treatment CBCT only once weekly for additional imaging information. Implications for practice: Further research is necessary to assess other uncertainties, to enable the calculation of a margin and determining the feasibility of further reduction of this. Crown Copyright (c) 2024 Published by Elsevier Ltd on behalf of The College of Radiographers. All rights reserved.