Drought stress induces early flowering and the stress tolerance of offspring in Petunia hybrida

Petunia hybrida (Solanaceae) exhibits high sensitivity to water scarcity, especially during flowering. This study investigated changes in the flowering time of P. hybrida in response to water deficit over a 7-week period. Various levels of water stress-i.e., light, moderate, and severe-were imposed on plants grown in a greenhouse, and these were compared to a control group grown alongside. Remarkably, early flowering was observed under severe stress in P. hybrida for the first time, occurring 5.3 days earlier than in the control group. Furthermore, seeds collected from control and treatment plants were then used to assess drought stress memory in offspring. Seedlings were cultivated in a dehydration medium containing either PEG 8000 or a control MS medium. In the PEG 8000 medium, seedlings from parents exposed to moderate and severe drought stresses exhibited higher drought tolerance than those from well-watered conditions. Moreover, they also displayed significantly longer roots, more leaves, and a lower ion leakage rate. Taken together, these findings demonstrated the presence of positive transgenerational effects on progeny. Thus, while parental drought stress during reproduction stage may affect seed quality, it can enhance drought tolerance in the next generation via induction of stress memory.