Genomic Assessment of Potential Probiotic Lactiplantibacillus plantarum CRM56-2 Isolated from Fermented Tea Leaves

被引：2
作者：
Kingkaew, Engkarat [1 ,2 ]
Tanaka, Naoto [3 ]
Shiwa, Yuh [3 ]
Sitdhipol, Jaruwan [4 ]
Nuhwa, Rattanatda [1 ]
Tanasupawat, Somboon [1 ]
机构：
[1] Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Biochem & Microbiol, Bangkok 10330, Thailand
[2] King Mongkuts Inst Technol Ladkrabang, Sch Sci, Dept Biol, Bangkok 10520, Thailand
[3] Tokyo Univ Agr, Fac Life Sci, Dept Mol Microbiol, 1-1-1 Sakuragaoka,Setagaya Ku, Tokyo 1568502, Japan
[4] Thailand Inst Sci & Technol Res, Biodivers Res Ctr, Res & Dev Grp Bioind, Pathum Thani 12120, Thailand
来源：
TROPICAL LIFE SCIENCES RESEARCH | 2024年 / 35卷 / 02期
关键词：
Lactiplantibacillus plantarum; Fermented Tea Leaves; Genome Sequencing; Safety Evaluation; Probiotic Properties; Cholesterol-Lowering Activity; LACTIC-ACID BACTERIA; LACTOBACILLUS-PLANTARUM; STRAIN;
D O I：
10.21315/tlsr2024.35.2.12
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Lactiplantibacillus plantarum is a widely studied species known for its probiotic properties that can help alleviate serum cholesterol levels. Whole-genome sequencing provides genetic information on probiotic attributes, metabolic activities and safety assessment. This study investigates the probiotic properties of strain CRM56-2, isolated from Thai fermented tea leaves, using Whole-Genome Sequencing (WGS) to evaluate the safety, health-promoting genes and functional analysis. Strain CRM56-2 showed bile salt hydrolase (BSH) activity, assimilated cholesterol at a rate of 75.94%, tolerated acidic and bile environments and attached to Caco-2 cells. Based on ANIb (98.9%), ANIm (99.2%), and digital DNA-DNA hybridisation (98.3%), strain CRM56-2 was identified as L. plantarum. . In silico analysis revealed that it was not pathogenic and contained no antibiotic resistance genes or plasmids. L. plantarum CRM56-2 possessed genes linked to several probiotic properties and beneficial impacts. The genome of strain CRM56-2 suggested that L. plantarum CRM56-2 is non-hazardous, with potential probiotic characteristics and beneficial impacts, which could enhance its probiotic application. Consequently, L. plantarum CRM56-2 demonstrated excellent cholesterol-lowering activity and probiotic properties.
引用
收藏
页码：249 / 269
页数：22
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