Progress and limitations in engineering cellular adhesion for research and therapeutics

被引：3

作者：

Chao, George ^{[1
]}

Zukin, Stefan ^{[2
]}

Fortuna, Patrick R. J. ^{[2
]}

Boettner, Benjamin ^{[2
]}

Church, George M. ^{[1
,2
]}

机构：

[1] Harvard Med Sch, Dept Genet, Boston, MA 02115 USA

[2] Harvard Med Sch, Wyss Inst, Boston, MA 02115 USA

来源：

TRENDS IN CELL BIOLOGY | 2024年 / 34卷 / 04期

关键词：

CLICK CHEMISTRY; BLOOD-VESSELS; ORGANOIDS; DISEASE; DNA; MICROTISSUES; TISSUES; CELLS; ATLAS; LIVER;

D O I：

10.1016/j.tcb.2023.07.007

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Intercellular interactions form the cornerstone of multicellular biology. Despite advances in protein engineering, researchers artificially directing physical cell interactions still rely on endogenous cell adhesion molecules (CAMs) alongside off-target interactions and unintended signaling. Recently, methods for directing cellular interactions have been developed utilizing programmable domains such as coiled coils (CCs), nanobody-antigen, and single-stranded DNA (ssDNA). We first discuss desirable molecular- and systems-level properties in engineered CAMs, using the helixCAM platform as a benchmark. Next, we propose applications for engineered CAMs in immunology, developmental biology, tissue engineering, and neuroscience. Biologists in various fields can readily adapt current engineered CAMs to establish control over cell interactions, and their utilization in basic and translational research will incentivize further expansion in engineered CAM capabilities.

引用

页码：277 / 287

页数：11

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