Protocol for isolating and identifying small extracellular vesicles derived from human umbilical cord mesenchymal stem cells

被引：1
|
作者：
Chen, Yanxia [1 ,2 ]
Qian, Hui [1 ,2 ,3 ]
Mak, Michael [4 ]
Tao, Zhimin [1 ,2 ,3 ,5 ]
机构：
[1] Jiangsu Univ, Sch Med, Jiangsu Key Lab Med Sci, Zhenjiang 212013, Jiangsu, Peoples R China
[2] Jiangsu Univ, Sch Med, Dept Lab Med, Lab Med, Zhenjiang 212013, Jiangsu, Peoples R China
[3] Jiangsu Univ, Zhenjiang Key Lab High Technol Res Exosomes Fdn &, Sch Med, Zhenjiang 212013, Jiangsu, Peoples R China
[4] Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA
[5] Jiangsu Univ, Affiliated Hosp, Dept Emergency Med, Zhenjiang 212001, Jiangsu, Peoples R China
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 03期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1016/j.xpro.2024.103197
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Small extracellular vesicles (sEVs) are lipid bilayer-enclosed particles secreted by living cells. Here, we present a protocol for the collection and isolation of sEVs derived from human umbilical cord mesenchymal stem cells (hucMSCs). We describe steps for characterizing their morphology and integrity by transmission electron microscopy (TEM) and size distribution using nanoparticle tracking analysis (NTA) and an atomic force microscope (AFM). We then detail procedures for assessing nanoparticle size analysis and molecular markers by western blotting and Flow NanoAnalyzer.
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页数：12
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