Exploring the genetic basis of resistance to Neopestalotiopsis species in strawberry

被引:2
作者
Alam, Elissar [1 ]
Moyer, Catalina [2 ]
Verma, Sujeet [3 ]
Peres, Natalia A. [4 ]
Whitaker, Vance M. [1 ]
机构
[1] Univ Florida, Inst Food & Agr Sci, Plant Breeding Grad Program, Gainesville, FL 32611 USA
[2] Univ Florida, IFAS Gulf Coast Res & Educ Ctr, Hort Sci Dept, Wimauma, FL USA
[3] Fall Creek Farm & Nursery Inc, Lowell, OR USA
[4] Univ Florida, IFAS Gulf Coast Res & Educ Ctr, Plant Pathol Dept, Wimauma, FL USA
基金
美国食品与农业研究所;
关键词
RECIPROCAL INTROGRESSION LINES; CELL-DEATH; 1ST REPORT; PLANT; PREDICTION; ASSOCIATION; SELECTION; ROT; PESTALOTIOPSIS; BIOSYNTHESIS;
D O I
10.1002/tpg2.20477
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Aggressive strains of Neopestalotiopsis sp. have recently emerged as devastating pathogens of strawberry (Fragaria x ananassa Duchesne ex Rozier), infecting nearly all plant parts and causing severe outbreaks of leaf spot and fruit rot in Florida and globally. The development of host resistance is imperative due to the absence of fungicides that effectively inhibit Neopestalotiopsis sp. growth on an infected strawberry crop. Here, we analyzed 1578 individuals from the University of Florida's (UF) strawberry breeding program to identify and dissect genetic variation for resistance to Neopestalotiopsis sp. and to explore the feasibility of genomic selection. We found that less than 12% of elite UF germplasm exhibited resistance, with narrow-sense heritability estimates ranging from 0.28 to 0.69. Through genome-wide association studies (GWAS), we identified two loci accounting for 7%-16% of phenotypic variance across four trials and 3 years. Several candidate genes encoding pattern recognition receptors, intra-cellular nucleotide-binding leucine-rich repeats, and downstream components of plant defense pathways co-localized with the Neopestalotiopsis sp. resistance loci. Interestingly, favorable alleles at the largest-effect locus were rare in elite UF material and had previously been unintentionally introduced from an exotic cultivar. The array-based markers and candidate genes described herein provide the foundation for targeting this locus through marker-assisted selection. The predictive abilities of genomic selection models, with and without explicitly modeling peak GWAS markers as fixed effects, ranged between 0.25 and 0.59, suggesting that genomic selection holds promise for enhancing resistance to Neopestalotiopsis sp. in strawberry.
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页数:17
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