Generation of human cerebral organoids with a structured outer subventricular zone

被引:9
作者
Walsh, Ryan M. [1 ]
Luongo, Raffaele [2 ,3 ]
Giacomelli, Elisa [1 ,5 ,6 ]
Ciceri, Gabriele [1 ]
Rittenhouse, Chelsea [1 ,4 ]
Verrillo, Antonietta [2 ,3 ]
Galimberti, Maura [5 ,6 ]
Bocchi, Vittoria Dickinson [1 ]
Wu, Youjun [7 ]
Xu, Nan [1 ,8 ]
Mosole, Simone [2 ,3 ]
Muller, James [9 ]
Vezzoli, Elena [5 ,6 ,12 ]
Jungverdorben, Johannes [1 ]
Zhou, Ting [7 ]
Barker, Roger A. [10 ,11 ]
Giacomelli, Elisa [1 ,5 ,6 ]
Studer, Lorenz [1 ,4 ]
Baggiolini, Arianna [2 ,3 ]
机构
[1] Ctr Stem Cell Biol & Dev Biol Program, Mem Sloan Kettering Canc Ctr, New York, NY 10065 USA
[2] Inst Oncol Res IOR, Bellinzona Inst Sci BIOS, CH-6500 Bellinzona, Switzerland
[3] Fac Biomed Sci, Univ Svizzera Italiana, CH-6900 Lugano, Switzerland
[4] Weill Cornell Med, Dept Neurosci, Grad Sch Med Sci, New York, NY 10065 USA
[5] Univ Milan, Dept Biosci, Lab Stem Cell Biol & Pharmacol Neurodegenerat Dis, I-20122 Milan, Italy
[6] Ist Nazl Genet Mol, INGM, I-20122 Milan, Italy
[7] Sloan Kettering Inst Canc Res, Ctr Stem Cell Biol & Dev Biol Program, The SKI Stem Cell Res Facil, New York, NY 10065 USA
[8] Louis V Gerstner Jr Grad Sch Biomed Sci, New York, NY 10065 USA
[9] Sloan Kettering Inst, Dev Biol & Immunol Programs, New York, NY 10065 USA
[10] Univ Cambridge, Cambridge Stem Cell Inst, Cambridge, England
[11] Univ Cambridge, John van Geest Ctr Brain Repair, Dept Clin Neurosci, Forvie Site, Cambridge, England
[12] Ist Sci San Raffaele, ALEMB Adv Light & Electron Microscopy BioImaging C, DIBIT 1, I-20132 Milan, Italy
来源
CELL REPORTS | 2024年 / 43卷 / 04期
基金
芬兰科学院; 英国惠康基金;
关键词
LEUKEMIA INHIBITORY FACTOR; PLURIPOTENT STEM-CELLS; RADIAL GLIA; DIFFERENTIATION; IDENTITY; EXPRESSION; NEURONS; SPECIFICATION; PROGENITORS; PLATFORM;
D O I
10.1016/j.celrep.2024.114031
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development and expansion. By activating the STAT3 signaling pathway using leukemia inhibitory factor (LIF), which is not expressed in guided cortical organoids, we define a cortical organoid differentiation method from human pluripotent stem cells (hPSCs) that recapitulates the expansion of a progenitor pool into the oSVZ. The oSVZ comprises progenitor cells expressing specific oRG markers such as GFAP , LIFR , and HOPX , closely matching human fetal oRG. Finally, incorporating neural crest -derived LIF-producing cortical pericytes into cortical organoids recapitulates the effects of LIF treatment. These data indicate that increasing the cellular complexity of the organoid microenvironment promotes the emergence of oRG and supports a platform to study oRG in hPSC-derived brain organoids routinely.
引用
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页数:25
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共 71 条
  • [1] LIF signaling regulates outer radial glial to interneuron fate during human cortical development
    Andrews, Madeline G.
    Siebert, Clara
    Wang, Li
    White, Matthew L.
    Ross, Jayden
    Morales, Raul
    Donnay, Megan
    Bamfonga, Gradi
    Mukhtar, Tanzila
    Mckinney, Arpanaarjun
    Gemenes, Kaila
    Wang, Shaohui
    Bi, Qiuli
    Crouch, Elizabeth E.
    Parikshak, Neelroop
    Panagiotakos, Georgia
    Huang, Eric
    Bhaduri, Aparna
    Kriegstein, Arnold R.
    [J]. CELL STEM CELL, 2023, 30 (10) : 1382 - +
  • [2] mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex
    Andrews, Madeline G.
    Subramanian, Lakshmi
    Kriegstein, Arnold R.
    [J]. ELIFE, 2020, 9
  • [3] Developmental chromatin programs determine oncogenic competence in melanoma
    Baggiolini, Arianna
    Callahan, Scott J.
    Montal, Emily
    Weiss, Joshua M.
    Trieu, Tuan
    Tagore, Mohita M.
    Tischfield, Sam E.
    Walsh, Ryan M.
    Suresh, Shruthy
    Fan, Yujie
    Campbell, Nathaniel R.
    Perlee, Sarah C.
    Saurat, Nathalie
    Hunter, Miranda, V
    Simon-Vermot, Theresa
    Huang, Ting-Hsiang
    Ma, Yilun
    Hollmann, Travis
    Tickoo, Satish K.
    Taylor, Barry S.
    Khurana, Ekta
    Koche, Richard P.
    Studer, Lorenz
    White, Richard M.
    [J]. SCIENCE, 2021, 373 (6559) : 1104 - +
  • [4] Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro
    Bardy, Cedric
    van den Hurk, Mark
    Eames, Tameji
    Marchand, Cynthia
    Hernandez, Ruben V.
    Kellogg, Mariko
    Gorris, Mark
    Galet, Ben
    Palomares, Vanessa
    Brown, Joshua
    Bang, Anne G.
    Mertens, Jerome
    Boehnke, Lena
    Boyer, Leah
    Simon, Suzanne
    Gage, Fred H.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (20) : E2725 - E2734
  • [5] Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain
    Bauer, Sylvian
    Patterson, Paul H.
    [J]. JOURNAL OF NEUROSCIENCE, 2006, 26 (46) : 12089 - 12099
  • [6] Generalizing RNA velocity to transient cell states through dynamical modeling
    Bergen, Volker
    Lange, Marius
    Peidli, Stefan
    Wolf, F. Alexander
    Theis, Fabian J.
    [J]. NATURE BIOTECHNOLOGY, 2020, 38 (12) : 1408 - 1414
  • [7] Cell stress in cortical organoids impairs molecular subtype specification
    Bhaduri, Aparna
    Andrews, Madeline G.
    Mancia Leon, Walter
    Jung, Diane
    Shin, David
    Allen, Denise
    Jung, Dana
    Schmunk, Galina
    Haeussler, Maximilian
    Salma, Jahan
    Pollen, Alex A.
    Nowakowski, Tomasz J.
    Kriegstein, Arnold R.
    [J]. NATURE, 2020, 578 (7793) : 142 - +
  • [8] SPECIFICITY OF GLIAL FIBRILLARY ACIDIC PROTEIN FOR ASTROGLIA
    BIGNAMI, A
    DAHL, D
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1977, 25 (06) : 466 - 469
  • [9] Pericytes and Neurovascular Function in the Healthy and Diseased Brain
    Brown, Lachlan S.
    Foster, Catherine G.
    Courtney, Jo-Maree
    King, Natalie E.
    Howells, David W.
    Sutherland, Brad A.
    [J]. FRONTIERS IN CELLULAR NEUROSCIENCE, 2019, 13
  • [10] Integrating single-cell transcriptomic data across different conditions, technologies, and species
    Butler, Andrew
    Hoffman, Paul
    Smibert, Peter
    Papalexi, Efthymia
    Satija, Rahul
    [J]. NATURE BIOTECHNOLOGY, 2018, 36 (05) : 411 - +