The Efficacy of Local Autologous Platelet-Rich Plasma Prepared by Single and Double Spin Methods in the Treatment of Chronic Ulcer

Background: Chronic nonhealing ulcers present significant challenges in diabetic, dermatological, and surgical patients. Platelet-rich plasma (PRP), enriched with bioactive factors, offers promise for wound healing enhancement. This study evaluates PRP's efficacy, prepared via single and double spin methods in nonhealing chronic ulcers. Methods: Twenty-two patients aged 18-65 years participated and 100 mL of blood was drawn into citrate phosphate dextrose adenine (CPDA) bags with all aseptic precautions. PRP was prepared by single and double spin methods. Patient serum and 10% calcium gluconate were added to fibrin gel. PRP was injected around the ulcer and then dressed. Dressings were changed on the fifth, 15th, and 20th days with PRP. Evaluation occurred on day 30 using surface area and volume assessments by both methods. Results: The single spin PRP group and double spin PRP group had 11 patients each with hemoglobin range of 10.79 +/- 1.88 to 12.63 +/- 2.22 g/dL. Initial lesions (16.27 cm(2)) significantly reduced to 14.76 cm(2) after double spin PRP sessions (p=0.005) and Initial lesions (9.87 cm(2)) significantly reduced to 7.65 cm(2) after single spin PRP sessions (p=0.005). Platelet count differences between whole blood and PRP were significant (p<0.05). Conclusions: The single spin PRP method exhibited considerable improvements in healing parameters, showcasing its potential for chronic ulcer management.