Antifungal susceptibility and multilocus sequence typing (MLST) of Cryptococcus neoformans complex from HIV-associated cryptococcal meningitis patients in Manaus, Amazonas, Brazil

被引：1

作者：

Alves, Marla Jalene ^{[1
,2
]}

Cruz, Katia Santana ^{[3
]}

Alves, Gleica Soyan Barbosa ^{[4
]}

Grisolia, Maria Eduarda ^{[1
]}

Menescal, Victoria Violeta Fernandes ^{[1
]}

do Nascimento, Izabella Sadalla ^{[1
]}

Menescal, Lizandra Stephanny Fernandes ^{[3
]}

Pinheiro, Silviane Bezerra ^{[5
,6
]}

da Silva, Felipe Almeida ^{[7
]}

Trilles, Luciana ^{[7
]}

de Souza, Joao Vicente Braga ^{[5
]}

Lazera, Marcia dos Santos ^{[2
,7
]}

Jackisch-Matsuura, Ani Beatriz ^{[1
]}

机构：

[1] Fiocruz MS, Lab Diversidade Microbiana Amazonia Importancia Sa, Inst Leonidas & Maria Deane, Manaus, Amazonas, Brazil

[2] Fiocruz MS, Programa Posgrad Med Trop, Convenio ILMD, IOC, Manaus, Amazonas, Brazil

[3] Fundacao Med Trop Dr Heitor Vieira Dourado, Lab Micol Med, Manaus, Amazonas, Brazil

[4] Univ Fed Amazonas UFAM, Inst Ciencias Exatas & Tecnol, Itacoatiara, Amazonas, Brazil

[5] Inst Nacl Pesquisas Amazonia INPA, Lab Micol, Manaus, Amazonas, Brazil

[6] Inst Nacl Pesquisas Amazonia INPA, Posgrad Genet Conservacao & Biol Evolut, Manaus, Amazonas, Brazil

[7] Fiocruz MS, Inst Nacl Infectol Evandro Chagas, Lab Micol, Rio De Janeiro, Brazil

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2024年 / 55卷 / 03期

关键词：

Cryptococcosis; Cryptococcus; HIV/AIDS; Antifungals; MLST; Amazonas; GATTII SPECIES COMPLEX; EPIDEMIOLOGIC CUTOFF VALUES; END-POINT DISTRIBUTIONS; PRACTICE GUIDELINES; AMPHOTERICIN-B; STATE; FLUCONAZOLE; MANAGEMENT; AIDS;

D O I：

10.1007/s42770-024-01378-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cryptococcus neoformans is primarily responsible for cases of cryptococcal meningitis in individuals with HIV/AIDS. This study evaluated the susceptibility of C. neoformans obtained from individuals with cryptococcal meningitis associated with HIV/AIDS in Manaus, Amazonas, Brazil, against the action of the antifungals amphotericin B, flucytosine, fluconazole, itraconazole and posaconazole and analyzed it using Multilocus Sequence Typing (MLST) in order to identify the Sequence Types (STs). We analyzed 30 isolates of C. neoformans, from 24 HIV/AIDS patients diagnosed with cryptococcosis from 2017 to 2019 in a reference hospital, in addition to 3 environmental isolates: 1 isolate obtained in the home of a patient and 2 isolates obtained from neighboring homes of patients. 86.6% (n = 26/30) of the clinical isolates were identified as C. neoformans VNI ST93, 6.6% (n = 2/30) as C. neoformans VNI ST5, 3.3% (n = 1/30) as C. neoformans VNI ST32 and 3.3% (n = 1/30) as C. neoformans VNB ST232. The environmental isolates were identified as C. neoformans VNI ST93 (n = 3/3). 96.6% (n = 29/30) isolates were sensitive to amphotericin B, though there was variation in the MIC. 60% (n = 18/30) presented a MIC above the proposed epidemiological cutoff values for one or more antifungals. All environmental isolates were sensitive to the tested antifungals. The MLST showed that there is an important relationship between C. neoformans VNI ST93 and individuals with HIV/AIDS, including in the environmental isolates analyzed. C. neoformans VNB ST232 was observed for the first time in Amazonas. Amphotericin B was proven to be the best drug, but fluconazole and posaconazole also showed relevant action.

引用

页码：2603 / 2611

页数：9