Determination of Flavonoids and Phenolic Acids in the Liver of Wistar Rats after a Dietary Enrichment with Corinthian Currant (Vitis vinifera L., var. Apyrena): A Liquid Chromatography-Tandem Mass Spectrometry Study

被引:1
作者
Vasilakopoulou, Paraskevi B. [1 ]
Yanni, Amalia E. [1 ]
Fanarioti, Eleni [2 ]
Dermon, Catherine R. [2 ]
Karathanos, Vaios T. [1 ,3 ]
Chiou, Antonia [1 ]
机构
[1] Harokopio Univ, Dept Dietet & Nutr, Lab Chem Biochem Phys Chem Foods, Kallithea 17676, Greece
[2] Univ Patras, Dept Biol, Lab Human & Anim Physiol, Patras 26500, Greece
[3] Agr Cooperat Union Aegh, Aeghion 25100, Greece
关键词
liver; rats; Corinthian currant; LC-MS; flavonoids; Vitis vinifera L; TISSUE DISTRIBUTION; HEALTHY-SUBJECTS; BRAIN-TISSUE; METABOLITES; QUERCETIN; BIOAVAILABILITY; ACCUMULATION; POLYPHENOLS; HYDROLYSIS; EXTRACTION;
D O I
10.1021/acs.jafc.4c01654
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Corinthian currants are dried fruits produced from Vitis vinifera L. var. Apyrena grape. This study investigated the distribution of phenolic compounds in male Wistar rat livers following two distinct Corinthian currant long-term dietary intake protocols (3 and 10% w/w). Method optimization, comparing fresh and lyophilized tissues, achieved satisfactory recoveries (>70%) for most analytes. Enzymatic hydrolysis conditions (37 degrees C, pH 5.0) minimally affected phenolics, but enzyme addition showed diverse effects. Hydrolyzed lyophilized liver tissue from rats consuming Corinthian currants (3 and 10% w/w) exhibited elevated levels of isorhamnetin (20.62 +/- 2.27 ng/g tissue and 33.80 +/- 1.38 ng/g tissue, respectively), along with similar effects for kaempferol, quercetin, and chrysin after prolonged Corinthian currant intake. This suggests their presence as phase II metabolites in the fasting-state liver. This study is the first to explore phenolic accumulation in rat liver, simulating real conditions of dried fruit consumption, as seen herein with Corinthian currant.
引用
收藏
页码:11549 / 11560
页数:12
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