Palladium/Platinum/Ruthenium Trimetallic Dendritic Nanozymes Exhibiting Enhanced Peroxidase-like Activity for Signal Amplification of Lateral Flow Immunoassays

被引：10
作者：
Wang, Weiguo [1 ]
Cao, Qianqian [1 ]
He, Jian [1 ]
Xie, Yafeng [2 ]
Zhang, Ying [3 ]
Yang, Lin [1 ]
Duan, Ming-Hui [4 ]
Wang, Jikai [1 ]
Li, Wei [1 ]
机构：
[1] Univ South China, Affiliated Nanhua Hosp, Inst Pharm & Pharmacol, Hengyang Med Sch,Dept Hepatobiliary Surg, Hengyang 421000, Hunan, Peoples R China
[2] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Clin Lab, Hengyang 421000, Hunan, Peoples R China
[3] Liaoning Inst Sci & Technol, Sch Biomed & Chem Engn, Benxi 117004, Peoples R China
[4] Univ South China, Inst Pathogen Biol, Hengyang Med Sch, Hengyang 421000, Hunan, Peoples R China
来源：
NANO LETTERS | 2024年 / 24卷 / 27期
关键词：
trimetallic; nanozyme; peroxidase-like activity; LFIA; signal amplification; PD-PT; NANOPARTICLES; GOLD; PLATINUM; SILVER;
D O I：
10.1021/acs.nanolett.4c01568
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Developing ultrasensitive lateral flow immunoassays (LFIAs) has garnered significant attention in the field of point-of-care testing. In this study, a trimetallic dendritic nanozyme (Pd@Pt-Ru) was synthesized through Ru deposition on a Pd@Pt core and utilized to enhancing the sensitivity of LFIAs. Pd@Pt-Ru exhibited a K-m value of 5.23 mM for detecting H2O2, which indicates an H2O2 affinity comparable with that of horseradish peroxidase. The Ru surface layer reduces the activation energy barrier, which increases the maximum reaction rate. As a proof of concept, the proposed Pd@Pt-Ru nanozyme was incorporated into LFIAs (A-Pd@Pt-Ru-LFIAs) for detecting human chorionic gonadotropin (hCG). Compared with conventional gold nanoparticle (AuNP)-LFIAs, A-Pd@Pt-Ru-LFIAs demonstrated 250-fold increased sensitivity, thereby enabling a visible detection limit as low as 0.1 IU/L. True positive and negative rates both reached 100%, which renders the proposed Pd@Pt-Ru nanozyme suitable for detecting hCG in clinical samples.
引用
收藏
页码：8311 / 8319
页数：9
相关论文
共 52 条
[1]   Sensitive and simultaneous detection of multi-index lung cancer biomarkers by an NIR-II fluorescence lateral-flow immunoassay platform [J].
Ao, Lijiao ;
Liao, Tao ;
Huang, Liang ;
Lin, Shan ;
Xu, Kui ;
Ma, Jiangtao ;
Qiu, Shaorong ;
Wang, Xiangyu ;
Zhang, Qiqing .
CHEMICAL ENGINEERING JOURNAL, 2022, 436
[2]   Catalysis with Colloidal Ruthenium Nanoparticles [J].
Axet, M. Rosa ;
Philippot, Karine .
CHEMICAL REVIEWS, 2020, 120 (02) :1085-1145
[3]   Nanozyme-involved biomimetic cascade catalysis for biomedical applications [J].
Cai, Xiaoli ;
Jiao, Lei ;
Yan, Hongye ;
Wu, Yu ;
Gu, Wenling ;
Du, Dan ;
Lin, Yuehe ;
Zhu, Chengzhou .
MATERIALS TODAY, 2021, 44 :211-228
[4]   Fe-N-C single-atom nanozyme for ultrasensitive, on-site and multiplex detection of mycotoxins using lateral flow immunoassay [J].
Cai, Xinfa ;
Ma, Fei ;
Jiang, Jun ;
Yang, Xianglong ;
Zhang, Zhaowei ;
Jian, Zelang ;
Liang, Meijuan ;
Li, Peiwu ;
Yu, Li .
JOURNAL OF HAZARDOUS MATERIALS, 2023, 441
[5]   Recent Advancements in Enzyme-Based Lateral Flow Immunoassays [J].
Calabria, Donato ;
Calabretta, Maria Maddalena ;
Zangheri, Martina ;
Marchegiani, Elisa ;
Trozzi, Ilaria ;
Guardigli, Massimo ;
Michelini, Elisa ;
Di Nardo, Fabio ;
Anfossi, Laura ;
Baggiani, Claudio ;
Mirasoli, Mara .
SENSORS, 2021, 21 (10)
[6]   Lateral-flow enzyme immunoconcentration for rapid detection of Listeria monocytogenes [J].
Cho, Il-Hoon ;
Irudayaraj, Joseph .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (10) :3313-3319
[7]   Monodispersed Ruthenium Nanoparticles on Nitrogen-Doped Reduced Graphene Oxide for an Efficient Lithium-Oxygen Battery [J].
Dai, Wenrui ;
Liu, Yuan ;
Wang, Meng ;
Lin, Ming ;
Lian, Xu ;
Luo, Yani ;
Yang, Jinlin ;
Chen, Wei .
ACS APPLIED MATERIALS & INTERFACES, 2021, 13 (17) :19915-19926
[8]   Oxygen Reduction Electrocatalyst of Pt on Au Nanoparticles through Spontaneous Deposition [J].
Dai, Yu ;
Chen, Shengli .
ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (01) :823-829
[9]   Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives [J].
Di Nardo, Fabio ;
Chiarello, Matteo ;
Cavalera, Simone ;
Baggiani, Claudio ;
Anfossi, Laura .
SENSORS, 2021, 21 (15)
[10]   Sensitive Lateral Flow Immunoassay Based on Specific Peptide and Superior Oxidase Mimics with a Universal Dual-Mode Significant Signal Amplification [J].
Dong, Tao ;
Zhang, Xin ;
Yuan, Jinlong ;
Lin, Ziting ;
Yin, Pengxue ;
Yu, Haipeng ;
Wang, Mingyang ;
Liu, Aihua .
ANALYTICAL CHEMISTRY, 2023, 95 (33) :12532-12540
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