Exogenous 5-Aminolevulinic acid improved low-temperature tolerance tomato seedling by regulating starch content and phenylalanine metabolism

被引:3
作者
Zhang, Zhengda [1 ,2 ,3 ]
Dang, Jiao [1 ,2 ,3 ]
Yuan, Luqiao [1 ,2 ,3 ]
Zhang, Yuhui [1 ,2 ,3 ]
Zhou, Fan [1 ]
Li, Tianlai [4 ]
Hu, Xiaohui [1 ,2 ,3 ]
机构
[1] Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China
[2] Minist Agr & Rural Affairs, Key Lab Protected Hort Engn Northwest, Yangling 712100, Shaanxi, Peoples R China
[3] Shaanxi Protected Agr Res Ctr, Yangling 712100, Shaanxi, Peoples R China
[4] Shenyang Agr Univ, Coll Hort, Shenyang 110866, Peoples R China
关键词
Tomato; Low temperature; 5-Aminolevulinic acid; Starch; Phenylalanine metabolism; TRANSCRIPTION FACTOR; COLD TOLERANCE; STRESS; RESPONSES; GROWTH; SUGAR;
D O I
10.1016/j.plaphy.2023.108083
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Tomato is an important horticultural cash crop, and low-temperature stress has seriously affected the yield and quality of tomato. 5-Aminolevulinic acid (ALA) is widely used in agriculture as an efficient and harmless growth regulator. It is currently unclear whether exogenous ALA can cope with low-temperature stress by regulating tomato starch content and phenylalanine metabolism. In this study, exogenous ALA remarkably improved the low-temperature tolerance of tomato seedlings. RNA-sequencing results showed that exogenous ALA affected starch metabolism and phenylalanine metabolism in tomato seedling leaves under low-temperature stress. Subsequently, we used histochemical staining, observation of chloroplast microstructure, substance content determination, and qRT-PCR analysis to demonstrate that exogenous ALA could improve the low-temperature tolerance of tomato seedlings by regulating starch content and phenylalanine metabolism (SlPAL, SlPOD1, and SlPOD2). Simultaneously, we found that exogenous ALA induced the expression of SlMYBs and SlWRKYs under low-temperature stress. In addition, dual luciferase, yeast one hybrid, and electrophoretic mobility shift assays indicate that SlMYB4 and SlMYB88 could regulate the expression of SlPOD2 in phenylalanine metabolism. We demonstrated that exogenous ALA could improve the low-temperature tolerance of tomato seedlings by regulating starch content and phenylalanine metabolism.
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页数:10
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