Absolute CD4 count and percentage values among Libyan patients with HIV by single-platform flow cytometry

Background Single-platform flow cytometry technology together with CD45-gating is becoming the method of choice for absolute CD4 T cell enumeration. Immunological assessment of HIV patients by monitoring CD4 can provide valuable information on antiviral treatment response and disease progression. Methods A total of 97 HIV-positive individuals were recruited from 2 hospitals in Tripoli, Libya, and 14 healthy blood donors. The HIV-infected individuals were classified by CD4+ count into HIV-positive (>200 cells/mu L) or AIDS (<= 200 cells/mu L) groups. CD4+ and CD8+ cell counts were determined and compared among the groups and with similar published data. Results The mean +/- SD CD4+ cell counts were 1106 +/- 442.8 cells/mu L in healthy individuals, 460 +/- 219.7 cells/mu L in the HIV-positive group, and 78 +/- 64.3 cells/mu L in the AIDS group. The mean +/- SD CD4+/CD8+ ratio was 1.6 +/- 0.58, 0.4 +/- 0.22, and 0.1 +/- 0.1, respectively. CD4+ counts in Libyan healthy adults might be higher than those reported in several studies in other regions, whereas CD4+ counts in Libyan AIDS patients seem lower. Conclusion Reference values for T lymphocyte counts in Libyan healthy individuals should be investigated more extensively, and the reasons why Libyan AIDS patients seem to have such lower CD4+ counts should be examined.