Three factors ParA, TipN, and DnaA-mediated chromosome replication initiation are contributors of centromere segregation in Caulobacter crescentus

被引:0
作者
Letzkus, Morgan [1 ]
Trela, Corey [1 ]
Mera, Paola E. [1 ]
机构
[1] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA
基金
美国国家卫生研究院;
关键词
P1; PARA; POLAR LOCALIZATION; BACILLUS-SUBTILIS; BINDING-SITES; CELL-DIVISION; F-PLASMID; PROTEIN; PARTITION; TRANSPORT; ANCHORS;
D O I
10.1091/mbc.E23-12-0503
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The ability of bacteria to maintain chromosomal integrity throughout their life cycle is crucial for survival. In Caulobacter crescentus, the polar factor TipN has been proposed to be involved with the partitioning system ParABS. Cells with tipN knocked out display subtle segregation defects of the centromere-like region parS. We hypothesized that TipN's role with parS segregation is obscured by other forces that are ParABS-independent. To test our hypothesis, we removed one of those forces - chromosome replication - and analyzed the role of TipN with ParA. We first confirm that ParA retains its ability to transport the centromeric region parS from the stalked pole to the opposite pole in the absence of chromosome replication. Our data revealed that in the absence of chromosome replication, TipN becomes essential for ParA's ability to transport parS. Furthermore, we identify a potential connection between the replication initiator DnaA and TipN. Although TipN is not essential for viability, tipN knockout cells lose viability when the regulation of DnaA levels is altered. Our data suggest that the DnaA-dependent susceptibility of tipN knockout cells is connected to parS segregation. Collectively, this work provides insights into the complex regulation involved in the coordination of chromosome replication and segregation in bacteria.
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页数:11
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