Autophagy-induced cell death by aqueous and polyphenol-enriched extracts of honeybush (Cyclopia spp.) in liver and colon cancer cells

被引：1

作者：

Samodien, Sedicka ^{[1
]}

de Kock, Maryna ^{[2
]}

Joubert, Elizabeth ^{[3
,4
]}

de Beer, Dalene ^{[3
,4
]}

Kriel, Jurgen ^{[5
]}

Gelderblom, Wentzel C. A. ^{[6
]}

Lilly, Mariska ^{[1
]}

机构：

[1] Cape Peninsula Univ Technol, Appl Microbial & Hlth Biotechnol Inst, POB 1906, ZA-7535 Bellville, South Africa

[2] Univ Western Cape, Dept Med Biosci Program, Bellville, South Africa

[3] Agr Res Council, Plant Bioact Grp, Postharvest & Agroproc Technol, Infruitec Nietvoorbij, Stellenbosch, South Africa

[4] Stellenbosch Univ, Dept Food Sci, Stellenbosch, South Africa

[5] Stellenbosch Univ, Electron Microscopy Unit, Cent Analyt Facil, Stellenbosch, South Africa

[6] Stellenbosch Univ, Dept Biochem, Stellenbosch, South Africa

来源：

FOOD SCIENCE & NUTRITION | 2024年 / 12卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

autophagy; cell proliferation; Cyclopia; mitochondrial integrity; polyphenols; OXIDATIVE STRESS; MITOCHONDRIAL DYSFUNCTION; PHENOLIC COMPOSITION; DIETARY POLYPHENOLS; APOPTOSIS; ANTIOXIDANT; MANGIFERIN; INDUCTION; GROWTH; ROS;

D O I：

10.1002/fsn3.4214

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The anti-cancer potential of Cyclopia species (honeybush) has been demonstrated in several models. The present study investigated the effects of aqueous and polyphenol-enriched (PE) extracts of C. subternata and C. genistoides, as well as mangiferin and hesperidin, on different cell growth parameters in human liver (HepG2) and colon (HT-29) cancer cells. Mangiferin and hesperidin were most abundant in C. genistoides and C. subternata, respectively. Cyclopia subternata extracts had the highest ferric-reducing antioxidant capacity. Following exposure of the cells to the extracts and compounds, cell viability, proliferation, and death (apoptosis and autophagy) were determined. Cyclopia subternata extracts reduced cell viability and inhibited cell proliferation the most, associated with depletion of ATP. In HepG2 cells, the PE extracts were less effective than the aqueous extracts in reducing cell viability but more effective in inhibiting cell proliferation. Despite disrupting cell growth, none of the extracts induced apoptosis. The aqueous extracts affected autophagy in both cancer cells. Disruption of mitochondrial membrane integrity by the different extracts, presumably via polyphenol/iron interactions, is postulated to be involved; however, mangiferin and hesperidin had no effect, suggesting that other polyphenols and/or complex interactions between compounds are likely responsible for the differential cytotoxic and/or cytoprotective effects of the extracts.

引用

页码：5647 / 5662

页数：16

共 63 条

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