Acute Effects of Dietary Protein Consumption on the Postprandial Metabolic Response, Amino Acid Levels and Circulating MicroRNAs in Patients with Obesity and Insulin Resistance

被引:1
作者
Hernandez-Gomez, Karla G. [1 ]
Velazquez-Villegas, Laura A. [1 ]
Granados-Portillo, Omar [1 ]
Avila-Nava, Azalia [2 ]
Gonzalez-Salazar, Luis E. [3 ]
Serralde-Zuniga, Aurora E. [3 ]
Palacios-Gonzalez, Berenice [4 ]
Pichardo-Ontiveros, Edgar [1 ]
Guizar-Heredia, Rocio [1 ]
Lopez-Barradas, Adriana M. [1 ]
Sanchez-Tapia, Monica [1 ]
Larios-Serrato, Violeta [5 ]
Olin-Sandoval, Viridiana [6 ]
Diaz-Villasenor, Andrea [7 ]
Medina-Vera, Isabel [8 ]
Noriega, Lilia G. [1 ]
Aleman-Escondrillas, Gabriela [1 ]
Ortiz-Ortega, Victor M. [1 ]
Torres, Nimbe [1 ]
Tovar, Armando R. [1 ]
Guevara-Cruz, Martha [1 ]
机构
[1] Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Dept Fisiol Nutr, Mexico City 14080, Mexico
[2] Hosp Reg Alta Especial Peninsula Yucatan, IMSS Bienestar, Merida 97130, Yucatan, Mexico
[3] Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Serv Nutriol Clin, Mexico City 14080, Mexico
[4] Ctr Invest Envejecimiento, Mexico City 14330, Mexico
[5] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Mexico City 11340, Mexico
[6] Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Biotecnol & Bioingn, Mexico City 07360, Mexico
[7] Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Med Genomica & Toxicol Ambiental, Mexico City 04510, Mexico
[8] Inst Nacl Pediat, Dept Metodol Invest, Mexico City 04530, Mexico
关键词
dietary protein; insulin resistance; obesity; amino acids; miRNAs; BETA-CELL FUNCTION; GLUCOSE-METABOLISM; SKELETAL-MUSCLE; PPAR-GAMMA; SECRETION; ASSOCIATION; GLUCAGON; LEUCINE; MIR-27A; RECEPTOR;
D O I
10.3390/ijms25147716
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The post-nutritional intervention modulation of miRNA expression has been previously investigated; however, post-acute dietary-ingestion-related miRNA expression dynamics in individuals with obesity and insulin resistance (IR) are unknown. We aimed to determine the acute effects of protein ingestion from different dietary sources on the postprandial metabolic response, amino acid levels, and circulating miRNA expression in adults with obesity and IR. This clinical trial included adults with obesity and IR who consumed (1) animal-source protein (AP; calcium caseinate) or (2) vegetable-source protein (VP; soy protein isolate). Glycaemic, insulinaemic, and glucagon responses, amino acid levels, and exosomal microRNAs isolated from plasma were analysed. Post-AP ingestion, the area under the curve (AUC) of insulin (p = 0.04) and the plasma concentrations of branched-chain (p = 0.007) and gluconeogenic (p = 0.01) amino acids increased. The effects of different types of proteins on the concentration of miRNAs were evaluated by measuring their plasma circulating levels. Compared with the baseline, the AP group presented increased circulating levels of miR-27a-3p, miR-29b-3p, and miR-122-5p (p < 0.05). Subsequent analysis over time at 0, 30, and 60 min revealed the same pattern and differences between treatments. We demonstrated that a single dose of dietary protein has acute effects on hormonal and metabolic regulation and increases exosomal miRNA expression in individuals with obesity and IR.
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页数:14
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