Two endo-1,4-β-glucanase gene family members from blackberry reveals divergent regulation during fruit ripening

被引:2
作者
Liu, Hongxia [1 ]
Wang, Xiaomin [1 ]
Lyu, Lianfei [1 ]
He, Jiaqi [2 ]
Wu, Wenlong [1 ]
Li, Weilin [2 ]
机构
[1] Jiangsu Prov & Chinese Acad Sci, Nanjing Bot Garden Mem Sun Yat Sen, Jiangsu Key Lab Res & Utilizat Plant Resources, Inst Bot, Qian Hu Hou Cun 1, Nanjing 210014, Peoples R China
[2] Nanjing Forestry Univ, Coll Forestry, Nanjing 210037, Peoples R China
关键词
RuEG; Firmness; Cell wall; Blackberry; CELL-WALL METABOLISM; EXPRESSION; POLYSACCHARIDES; PEROXIDASE; QUALITY; ENZYMES;
D O I
10.1016/j.scienta.2024.113256
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
As a new generation of economically important fruit, blackberry fruit is rich in nutrients and functional components. However, the ripe fruit is very low in firmness, soft, and perishable. Therefore, storage and preservation have become important factors restricting its industrial development. The overexpression vectors of RuEG1 and RuEG2 encoded endo-1,4-beta-glucanase from blackberry were introduced into tomato to identify their functions at different ripening stages. The results showed that firmness of transgenic tomato fruit decreased with the development period. The structure of transgenic tomato collapsed earlier than that of wild-type tomato. The comparison showed that the contents of cellulose, hemicellulose, and pectin in transgenic tomato were significantly reduced compared with wild-type tomato. Pectin methylesterase and cellulase activities were significantly increased in transgenic compared with wild-type tomato. Transcriptome and qRT-PCR data showed that the expression levels of cell wall degrading enzyme genes SlXTH3, SlCel2 and SlCel8 were significantly higher than those of the control. The genes of RuEG1 and RuEG2 regulated enzyme activity in fruit cell walls and influenced cell wall composition, thus regulating fruit firmness and improving fruit quality and storability. In addition, the SlXTH3 and SlCel8 genes were higher expressed in RuEG2 than in RuEG1 transgenic fruits at the yellow fruit stage, which might be the reason why they reveal divergent regulation during fruit ripening stages.
引用
收藏
页数:9
相关论文
共 34 条
[1]   Changes in pectic enzymes and cellulase activity during guava fruit ripening [J].
Abu-Goukh, ABA ;
Bashir, HA .
FOOD CHEMISTRY, 2003, 83 (02) :213-218
[2]   Molecular cloning and characterization of cel2 from the fungus Cochlibolus carbonum [J].
Ahn, JH ;
Sposato, P ;
Kim, SI ;
Walton, JD .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2001, 65 (06) :1406-1411
[3]   Analysis of xyloglucan endotransglucosylase/hydrolase (XTH) gene families in kiwifruit and apple [J].
Atkinson, Ross G. ;
Johnston, Sarah L. ;
Yauk, Yar-Khing ;
Sharma, Neelam N. ;
Schroder, Roswitha .
POSTHARVEST BIOLOGY AND TECHNOLOGY, 2009, 51 (02) :149-157
[4]   Cell wall metabolism during maturation, ripening and senescence of peach fruit [J].
Brummell, DA ;
Dal Cin, V ;
Crisosto, CH ;
Labavitch, JM .
JOURNAL OF EXPERIMENTAL BOTANY, 2004, 55 (405) :2029-2039
[5]   Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants [J].
Brummell, DA ;
Harpster, MH .
PLANT MOLECULAR BIOLOGY, 2001, 47 (1-2) :311-340
[6]  
Desantis D., 1988, Carbohydrate metabolism in Mollicutes: the Embden-MeyerhofParnas pathway and the hexose monophosphate shunt
[7]   Populus endo-β-1,4-glucanases gene family: genomic organization, phylogenetic analysis, expression profiles and association mapping [J].
Du, Qingzhang ;
Wang, Lu ;
Yang, Xiaohui ;
Gong, Chenrui ;
Zhang, Deqiang .
PLANTA, 2015, 241 (06) :1417-1434
[8]   ROLE OF CELL-WALL HYDROLASES IN FRUIT RIPENING [J].
FISCHER, RL ;
BENNETT, AB .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1991, 42 :675-703
[9]   Absence of the endo-β-1,4-glucanases Cel1 and Cel2 reduces susceptibility to Botrytis cinerea in tomato [J].
Flors, Victor ;
Leyva, Maria de la O. ;
Vicedo, Begonya ;
Finiti, Ivan ;
Real, Maria Dolores ;
Garcia-Agustin, Pilar ;
Bennett, Alan B. ;
Gonzalez-Bosch, Carmen .
PLANT JOURNAL, 2007, 52 (06) :1027-1040
[10]   Molecular characterization and heterologous expression of a Xanthophyllomyces dendrorhous α-glucosidase with potential for prebiotics production [J].
Gutierrez-Alonso, Patricia ;
Gimeno-Perez, Maria ;
Ramirez-Escudero, Mercedes ;
Plou, Francisco J. ;
Sanz-Aparicio, Julia ;
Fernandez-Lobato, Maria .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (07) :3125-3135