Tailorable mechanical and degradation properties of KCl-reticulated and BDDE-crosslinked PCL/chitosan/κ-carrageenan electrospun fibers for biomedical applications: Effect of the crosslinking-reticulation synergy

被引：0

作者：

Vargas-Osorio, Zulema ^{[1
,2
]}

Castillo, Eduin I. Gonzalez ^{[1
,2
,3
]}

Mutlu, Nurshen ^{[1
,2
]}

Vidomanov, Eva ^{[1
]}

Michalek, Martin ^{[1
]}

Galusek, Dusan ^{[1
,4
]}

Boccaccini, Aldo R. ^{[2
]}

机构：

[1] Alexander Dubcek Univ Trencin, Ctr Funct & Surface Functionalized Glass, Studentska 2, Trencin 91150, Slovakia

[2] Univ Erlangen Nurnberg, Inst Biomat, Dept Mat Sci & Engn, D-91058 Erlangen, Germany

[3] AO Res Inst Davos, Clavadelerstr 8, CH-7270 Davos, Switzerland

[4] TnUAD, Joint Glass Ctr IIC SAS, FChPT STU, Studentska 2, Trencin 91150, Slovakia

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 265卷

基金：

瑞士国家科学基金会;

关键词：

Crosslinked electrospun fibers; BDDE crosslinking agent; Reticulation with KCl; PCL/chitosan; kappa-Carrageenan; HYDROLYTIC DEGRADATION; HYALURONIC-ACID; CHITOSAN; POLYSACCHARIDE; NANOFIBERS; SCAFFOLDS; BLENDS;

D O I：

10.1016/j.ijbiomac.2024.130647

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of intricated and interconnected porous mats is desired for many applications in biomedicine and other relevant fields. The mats that comprise the use of natural, bioactive, and biodegradable polymers are the focus of current research activities. In the present work, crosslinked fibers with improved characteristics were produced by incorporating 1,4-butanediol diglycidyl ether (BDDE) into a polymer formulation containing polycaprolactone (PCL), chitosan (CS), and kappa appa-carrageenan ( kappa-C). A slight variation of formic acid (FA)/acetic acid (AA) ratio used as a solvent system, significantly affected the characteristics of the produced fiber mats. Both polysaccharides and BDDE played a major role in tailoring mechanical properties when fibrous scaffolds were reticulated under KCl-mediated basic conditions for determined periods of time at 50 degrees C. In vitro biological assessment of the electrospun fiber mats revealed proliferation of MC3T3-E1 cells when incubated for 1 and 7 days. After staining the cells with 4 ' ,6-diamidino-2-phenylindole (DAPI)/rhodamine phalloidin an autofluorescence response was observed by fluorescence microscopy in the scaffold manufactured using a solvent with higher FA/AA ratio due to the formation of microfibers. The results demonstrated the potential of the BDDEcrosslinked PCL/CS/ kappa-C electrospun fibers as promising materials for biomedical applications that may include soft and bone tissue regeneration.

引用

页数：13