Spinosad-induced intergenerational sublethal effects on Tuta absoluta : biological traits and related genes expressions

被引：15

作者：

Ullah, Farman ^{[1
]}

Guncan, Ali ^{[2
]}

Gul, Hina ^{[3
]}

Hafeez, Muhammad ^{[1
]}

Zhou, Shuxing ^{[1
]}

Wang, Yaru ^{[4
]}

Zhang, Zhijun ^{[1
]}

Huang, Jun ^{[1
]}

Ghramh, Hamed A. ^{[5
,6
]}

Guo, Wenchao ^{[7
]}

Fu, Kaiyun ^{[7
]}

Li, Xiaowei ^{[1
]}

Desneux, Nicolas ^{[8
]}

Lu, Yaobin ^{[1
,4
]}

机构：

[1] Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China

[2] Ordu Univ, Fac Agr, Dept Plant Protect, TR-52200 Ordu, Turkiye

[3] China Agr Univ, Coll Plant Protect, Dept Entomol, MARA Key Lab Pest Monitoring & Green Management, Beijing 100193, Peoples R China

[4] Xianghu Lab, Hangzhou 311231, Peoples R China

[5] King Khalid Univ, Unit Bee Res & Honey Prod, Res Ctr Adv Mat Sci RCAMS, Ctr Bee Res & Its Prod, POB 9004, Abha 61413, Saudi Arabia

[6] King Khalid Univ, Fac Sci, Biol Dept, POB 9004, Abha 61413, Saudi Arabia

[7] Xinjiang Acad Agr Sci, Inst Plant Protect, Key Lab Integrated Pest Management Crops Northwes, Xinjiang Key Lab Agr Biosafety Urumqi,Minist Agr, Xinjiang, Peoples R China

[8] Univ Cote Azur, INRAE, UMR ISA, F-06000 Nice, France

来源：

ENTOMOLOGIA GENERALIS | 2024年 / 44卷 / 02期

基金：

中国国家自然科学基金;

关键词：

biopesticide; South American tomato pinworm; insecticide toxicity; demographic parameters; age-stage; two- sex life table; LIFE; POPULATIONS; INSECTICIDE; LETHAL; APHID;

D O I：

10.1127/entomologia/2024/2452

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is considered one of the most economically important invasive pests that causes severe damage to tomato crops. The biopesticide spinosad is widely used for controlling T. absoluta . This study aimed to investigate the spinosad-induced intergenerational sublethal effects on T. absoluta . Results showed that spinosad exhibited high toxicity against 3 rd instar T. absoluta with the LC 50 of 0.04 mg L -1 after 72 h exposure. The LC 10 and LC 30 of spinosad significantly decreased the durations of various developmental stages and some reproductive parameters of parental (F 0 ) T. absoluta compared to control. The developmental durations, survival rate, reproduction and life table parameters (intrinsic rate of increase, r ; finite rate of increase, lambda ; net reproductive rate, R 0 ; and oviposition days, O d ) were significantly decreased in the progeny generations (F 1 and F 2 ) following F 0 exposure to the LC 10 and LC 30 of spinosad as compared to the control group. Additionally, the mRNA expression levels of development and reproduction related genes such as Vg, VgR , and JHBP were down-regulated in parental and progeny generations at both spinosad treatments as compared to control treatment. The resistance related cytochrome P450 genes CYP321C40 and CYP6AW1 , followed by CYP4M116, CYP9A307v2, CYP15C1, CYP339A1 , and CYP6AB327 were up-regulated at spinosad-treated groups in the parental and progeny generations of T. absoluta as compared to control. Overall, these results showed that although spinosad exhibited high toxicity and induces intergenerational sublethal effects on the parental and progeny generations, the indiscriminate and long-term application might causes resistance development in T. absoluta .

引用

页码：395 / 404

页数：10

共 37 条

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