Rift Valley Fever Phlebovirus Reassortment Study in Sheep

被引:1
作者
Balaraman, Velmurugan [1 ]
Indran, Sabarish V. [1 ,3 ]
Kim, In Joong [1 ,4 ]
Trujillo, Jessie D. [1 ]
Meekins, David A. [1 ]
Shivanna, Vinay [1 ,5 ]
Zajac, Michelle D. [1 ,2 ]
Urbaniak, Kinga [1 ,6 ]
Morozov, Igor [1 ]
Sunwoo, Sun-Young [1 ,7 ]
Faburay, Bonto [1 ,2 ]
Osterrieder, Klaus [1 ,8 ]
Gaudreault, Natasha N. [1 ]
Wilson, William C. [2 ]
Richt, Juergen A. [1 ]
机构
[1] Kansas State Univ, Coll Vet Med, Ctr Excellence Emerging & Zoonot Anim Dis Diagnost, Manhattan, KS 66506 USA
[2] USDA ARS, Foreign Arthropod Borne Anim Dis Res Unit, Natl Bio & Agrodef Facil, Manhattan, KS 66505 USA
[3] Sanofi, Swiftwater, PA 18370 USA
[4] Bristol Myers Squibb, Princeton, NJ 08540 USA
[5] Texas Biomed Res Inst, San Antonio, TX 78227 USA
[6] Natl Vet Res Inst, PL-24100 Pulawy, Poland
[7] Konkuk Univ, Coll Vet Med, 120 Neungdong Ro, Seoul 05029, South Korea
[8] Free Univ Berlin, Inst Virol, D-14195 Berlin, Germany
来源
VIRUSES-BASEL | 2024年 / 16卷 / 06期
关键词
Rift Valley fever phlebovirus; bunyavirus; sheep; reassortment; GENETIC DIVERSITY; MOLECULAR-BIOLOGY; CHALLENGE MODEL; TRANSMISSION; VACCINE;
D O I
10.3390/v16060880
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Rift Valley fever (RVF) in ungulates and humans is caused by a mosquito-borne RVF phlebovirus (RVFV). Live attenuated vaccines are used in livestock (sheep and cattle) to control RVF in endemic regions during outbreaks. The ability of two or more different RVFV strains to reassort when co-infecting a host cell is a significant veterinary and public health concern due to the potential emergence of newly reassorted viruses, since reassortment of RVFVs has been documented in nature and in experimental infection studies. Due to the very limited information regarding the frequency and dynamics of RVFV reassortment, we evaluated the efficiency of RVFV reassortment in sheep, a natural host for this zoonotic pathogen. Co-infection experiments were performed, first in vitro in sheep-derived cells, and subsequently in vivo in sheep. Two RVFV co-infection groups were evaluated: group I consisted of co-infection with two wild-type (WT) RVFV strains, Kenya 128B-15 (Ken06) and Saudi Arabia SA01-1322 (SA01), while group II consisted of co-infection with the live attenuated virus (LAV) vaccine strain MP-12 and a WT strain, Ken06. In the in vitro experiments, the virus supernatants were collected 24 h post-infection. In the in vivo experiments, clinical signs were monitored, and blood and tissues were collected at various time points up to nine days post-challenge for analyses. Cell culture supernatants and samples from sheep were processed, and plaque-isolated viruses were genotyped to determine reassortment frequency. Our results show that RVFV reassortment is more efficient in co-infected sheep-derived cells compared to co-infected sheep. In vitro, the reassortment frequencies reached 37.9% for the group I co-infected cells and 25.4% for the group II co-infected cells. In contrast, we detected just 1.7% reassortant viruses from group I sheep co-infected with the two WT strains, while no reassortants were detected from group II sheep co-infected with the WT and LAV strains. The results indicate that RVFV reassortment occurs at a lower frequency in vivo in sheep when compared to in vitro conditions in sheep-derived cells. Further studies are needed to better understand the implications of RVFV reassortment in relation to virulence and transmission dynamics in the host and the vector. The knowledge learned from these studies on reassortment is important for understanding the dynamics of RVFV evolution.
引用
收藏
页数:13
相关论文
共 50 条
  • [21] No evidence of Rift Valley fever antibodies in veterinarians and sheep in Northern Palestine
    Alzuheir, Ibrahim
    Abu Helal, Belal
    Abu Helal, Mohammad
    Fayyad, Adnan
    Jalboush, Nasr
    VETERINARY WORLD, 2022, 15 (08) : 1990 - 1995
  • [22] The first imported case of Rift Valley fever in China reveals a genetic reassortment of different viral lineages
    Liu, Jingyuan
    Sun, Yulan
    Shi, Weifeng
    Tan, Shuguang
    Pan, Yang
    Cui, Shujuan
    Zhang, Qingchao
    Dou, Xiangfeng
    Lv, Yanning
    Li, Xinyu
    Li, Xitai
    Chen, Lijuan
    Quan, Chuansong
    Wang, Qianli
    Zhao, Yingze
    Lv, Qiang
    Hua, Wenhao
    Zeng, Hui
    Chen, Zhihai
    Xiong, Haofeng
    Jiang, Chengyu
    Pang, Xinghuo
    Zhang, Fujie
    Liang, Mifang
    Wu, Guizhen
    Gao, George F.
    Liu, William J.
    Li, Ang
    Wang, Quanyi
    EMERGING MICROBES & INFECTIONS, 2017, 6
  • [23] Efficacy of Montanide (IMS 3015) as an adjuvant for an inactivated Rift Valley fever (RVF) vaccine in sheep
    Hussein, Mohamed A.
    Ali, Haytham A.
    Abd El-Rhman, Bahgat A.
    Morcoss, Timour N.
    Fakhr, Ahmed Elsadek
    Swelum, Ayman Abdel Aziz
    Alowaimer, Abdullah
    Tukur, Hammed A.
    Saadeldin, Islam M.
    ACTA TROPICA, 2019, 190 : 193 - 203
  • [24] Rift Valley fever vaccines
    Ikegami, Tetsuro
    Makino, Shinji
    VACCINE, 2009, 27 : D69 - D72
  • [25] Development of a Rift Valley fever virus viremia challenge model in sheep and goats
    Weingartl, Hana M.
    Miller, Myrna
    Nfon, Charles
    Wilson, William C.
    VACCINE, 2014, 32 (20) : 2337 - 2344
  • [26] Serological evidence of rift valley fever Phlebovirus and Crimean-Congo hemorrhagic fever orthonairovirus infections among pygmies in the east region of Cameroon
    Serge Alain Sadeuh-Mba
    Gide Martial Yonga Wansi
    Maurice Demanou
    Antoine Gessain
    Richard Njouom
    Virology Journal, 15
  • [27] Serological evidence of rift valley fever Phlebovirus and Crimean-Congo hemorrhagic fever orthonairovirus infections among pygmies in the east region of Cameroon
    Sadeuh-Mba, Serge Alain
    Wansi, Gide Martial Yonga
    Demanou, Maurice
    Gessain, Antoine
    Njouom, Richard
    VIROLOGY JOURNAL, 2018, 15
  • [28] Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats
    Balkema-Buschmann, Anne
    Rissmann, Melanie
    Kley, Nils
    Ulrich, Reiner
    Eiden, Martin
    Groschup, Martin H.
    VIRUSES-BASEL, 2018, 10 (12):
  • [29] Safety study of Rift Valley Fever human vaccine candidate (DDVax) in mosquitoes
    Campbell, Corey L.
    Snell, Trey K.
    Bennett, Susi
    Wyckoff, John H., III
    Heaslip, Darragh
    Flatt, Jordan
    Harris, Emma K.
    Hartman, Daniel A.
    Lian, Elena
    Bird, Brian H.
    Stenglein, Mark D.
    Bowen, Richard A.
    Kading, Rebekah C.
    TRANSBOUNDARY AND EMERGING DISEASES, 2022, 69 (05) : 2621 - 2633
  • [30] Lesions and Cellular Tropism of Natural Rift Valley Fever Virus Infection in Adult Sheep
    Odendaal, Lieza
    Clift, Sarah J.
    Fosgate, Geoffrey T.
    Davis, A. Sally
    VETERINARY PATHOLOGY, 2019, 56 (01) : 61 - 77