Co-Immobilization of Alcalase/Dispase for Production of Selenium-Enriched Peptide from Cardamine violifolia

被引:1
|
作者
Zhu, Shiyu [1 ]
Li, Yuheng [1 ]
Chen, Xu [1 ]
Zhu, Zhenzhou [1 ]
Li, Shuyi [1 ]
Song, Jingxin [2 ]
Zheng, Zhiqiang [2 ]
Cong, Xin [1 ]
Cheng, Shuiyuan [1 ]
机构
[1] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, 36 Huanhu Middle Rd, Wuhan 430048, Peoples R China
[2] Syst Engn Inst, Beijing 100010, Peoples R China
关键词
selenium; peptide; co-immobilization; enzyme; Cardamine violifolia; IMMOBILIZED ALCALASE; PROTEIN; ENZYMOLYSIS; HYDROLYSIS; LACCASE;
D O I
10.3390/foods13111753
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Enzymatically derived selenium-enriched peptides from Cardamine violifolia (CV) can serve as valuable selenium supplements. However, the industrial application of free enzyme is impeded by its limited stability and reusability. Herein, this study explores the application of co-immobilized enzymes (Alcalase and Dispase) on amino resin for hydrolyzing CV proteins to produce selenium-enriched peptides. The successful enzyme immobilization was confirmed through scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and Fourier-transform infrared spectroscopy (FTIR). Co-immobilized enzyme at a mass ratio of 5:1 (Alcalase/Dispase) exhibited the smallest pore size (7.065 nm) and highest activity (41 U/mg), resulting in a high degree of hydrolysis of CV protein (27.2%), which was obviously higher than the case of using free enzymes (20.7%) or immobilized Alcalase (25.8%). In addition, after a month of storage, the co-immobilized enzyme still retained a viability level of 41.93%, showing fairly good stability. Encouragingly, the selenium-enriched peptides from co-immobilized enzyme hydrolysis exhibited uniform distribution of selenium forms, complete amino acid fractions and homogeneous distribution of molecular weight, confirming the practicality of using co-immobilized enzymes for CV protein hydrolysis.
引用
收藏
页数:18
相关论文
共 42 条
  • [1] Production of Cardamine violifolia selenium-enriched peptide using immobilized Alcalase on Fe3O4 modified by tannic acid and polyethyleneimine
    Zhu, Shiyu
    Cong, Xin
    Sun, Zheng
    Chen, Zhe
    Chen, Xu
    Zhu, Zhenzhou
    Li, Shuyi
    Cheng, Shuiyuan
    RSC ADVANCES, 2022, 12 (34) : 22082 - 22090
  • [2] Antioxidant Activity of Selenium-Enriched Peptides from the Protein Hydrolysate of Cardamine violifolia
    Zhu, Song
    Du, Chaodong
    Yu, Tian
    Cong, Xin
    Liu, Yuanfa
    Chen, Shangwei
    Li, Yue
    JOURNAL OF FOOD SCIENCE, 2019, 84 (12) : 3504 - 3511
  • [3] Protective Effect of Selenium-enriched Peptide from Cardamine violifolia on Ethanol-induced L-02 Hepatocyte InjuryProtective Effect of Selenium-enriched Peptide from Cardamine violifolia on Ethanol-induced L-02 Hepatocyte InjuryLing et al.
    Rongrui Ling
    Chaodong Du
    Yue Li
    Shan Wang
    Xin Cong
    Dejian Huang
    Shangwei Chen
    Song Zhu
    Biological Trace Element Research, 2025, 203 (1) : 139 - 152
  • [4] Highly selective fluorescence detection of l-selenium-methylselenocysteine in selenium-enriched Cardamine violifolia
    Shi, Xiaoran
    Zhao, Hui
    Zhang, Han
    Li, Qunfang
    Lou, Fangming
    ANALYTICAL METHODS, 2024, 16 (26) : 4373 - 4380
  • [5] Protective Effect of Selenium-enriched Peptide from Cardamine violifolia on Ethanol-induced L-02 Hepatocyte Injury
    Ling, Rongrui
    Du, Chaodong
    Li, Yue
    Wang, Shan
    Cong, Xin
    Huang, Dejian
    Chen, Shangwei
    Zhu, Song
    BIOLOGICAL TRACE ELEMENT RESEARCH, 2025, 203 (01) : 139 - 152
  • [6] Selenium-Enriched Cardamine violifolia Increases Selenium and Decreases Cholesterol Concentrations in Liver and Pectoral Muscle of Broilers
    Zhao, Ling
    Chu, Xiao-Han
    Liu, Shuai
    Li, Rong
    Zhu, Yun-Fen
    Li, Feng-Na
    Jiang, Jie
    Zhou, Ji-Chang
    Lei, Xin Gen
    Sun, Lv-Hui
    JOURNAL OF NUTRITION, 2022, 152 (09): : 2072 - 2079
  • [7] Antioxidant and anti-fatigue activities of selenium-enriched peptides isolated from Cardamine violifolia protein hydrolysate
    Zhu, Song
    Yang, Wei
    Lin, Yue
    Du, Chaodong
    Huang, Dejian
    Chen, Shangwei
    Yu, Tian
    Cong, Xin
    JOURNAL OF FUNCTIONAL FOODS, 2021, 79
  • [8] Recovery of Selenium-Enriched Polysaccharides from Cardamine violifolia Residues: Comparison on Structure and Antioxidant Activity by Different Extraction Methods
    Liang, Yong
    Yu, Jiali
    Wu, Lulu
    Cong, Xin
    Liu, Haiyuan
    Chen, Xu
    Li, Shuyi
    Zhu, Zhenzhou
    ANTIOXIDANTS, 2024, 13 (10)
  • [9] Selenium-enriched peptides isolated from Cardamine violifolia are potent in suppressing proliferation and enhancing apoptosis of HepG2 cells
    Lin, Yue
    Li, Yue
    Cong, Xin
    Xia, Yongmei
    Huang, Dejian
    Chen, Shangwei
    Zhu, Song
    JOURNAL OF FOOD SCIENCE, 2022, 87 (07) : 3235 - 3247
  • [10] Selenium-enriched Cardamine violifolia improves growth performance with potential regulation of intestinal health and antioxidant function in weaned pigs
    Wang, Dan
    Zhang, Yue
    Chen, Qinliang
    Kuang, Yanling
    Fan, Jiajun
    Xu, Xiao
    Zhu, Huiling
    Gao, Qingyu
    Cheng, Shuiyuan
    Cong, Xin
    Liu, Yulan
    FRONTIERS IN VETERINARY SCIENCE, 2022, 9