Sugarcane bagasse derived xylooligosaccharides produced by an arabinofuranosidase/xylobiohydrolase from Bifidobacterium longum in synergism with xylanases

被引:2
作者
Capetti, Caio Cesar de Mello [1 ]
Ontanon, Ornella [2 ,3 ]
Navas, Laura E. [2 ,3 ]
Campos, Eleonora [2 ,3 ]
Simister, Rachael [4 ]
Dowle, Adam [5 ]
Liberato, Marcelo Vizona
de Oliveira, Vanessa
Pellegrini, Arnoldi
Gomez, Leonardo D. [4 ]
Polikarpov, Igor [1 ]
机构
[1] Univ Sao Paulo, Inst Fis Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP, Brazil
[2] Inst Nacl Tecnol Agr INTA, CICVyA, Inst Agrobiotecnol & Biol Mol IABIMO, Hurlingham B1686, Buenos Aires, Argentina
[3] Consejo Nacl Invest Cientif & Tecn CONICET, Buenos Aires, Argentina
[4] Univ York, Ctr Novel Agr Prod, Dept Biol, CNAP, York YO10 5DD, England
[5] Univ York, Dept Biol, Prote Lab, Technol Facil, York YO10 5DD, England
基金
英国生物技术与生命科学研究理事会;
关键词
Arabinofuranosidase; Xylooligosaccharides; Xylanases; Sugarcane bagasse; ALPHA-L-ARABINOFURANOSIDASES; GLYCOSIDE HYDROLASE FAMILY; ARABINOXYLAN ARABINOFURANOHYDROLASE; POLYSACCHARIDES; PURIFICATION; SPECIFICITY; COMPLEX; ACID;
D O I
10.1016/j.carbpol.2024.122248
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Arabinoxylan is a major hemicellulose in the sugarcane plant cell wall with arabinose decorations that impose steric restrictions on the activity of xylanases against this substrate. Enzymatic removal of the decorations by arabinofuranosidases can allow a more efficient arabinoxylan degradation by xylanases. Here we produced and characterized a recombinant Bifidobacterium longum arabinofuranosidase from glycoside hydrolase family 43 ( Bl Abf43) and applied it, together with GH10 and GH11 xylanases, to produce xylooligosaccharides (XOS) from wheat arabinoxylan and alkali pretreated sugarcane bagasse. The enzyme synergistically enhanced XOS production by GH10 and GH11 xylanases, being particularly efficient in combination with the latter family of enzymes, with a degree of synergism of 1.7. We also demonstrated that the enzyme is capable of not only removing arabinose decorations from the arabinoxylan and from the non -reducing end of the oligomeric substrates, but also hydrolyzing the xylan backbone yielding mostly xylobiose and xylose in particular cases. Structural studies of Bl Abf43 shed light on the molecular basis of the substrate recognition and allowed hypothesizing on the structural reasons of its multifunctionality.
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页数:13
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