Sugarcane bagasse derived xylooligosaccharides produced by an arabinofuranosidase/xylobiohydrolase from Bifidobacterium longum in synergism with xylanases
Arabinoxylan is a major hemicellulose in the sugarcane plant cell wall with arabinose decorations that impose steric restrictions on the activity of xylanases against this substrate. Enzymatic removal of the decorations by arabinofuranosidases can allow a more efficient arabinoxylan degradation by xylanases. Here we produced and characterized a recombinant Bifidobacterium longum arabinofuranosidase from glycoside hydrolase family 43 ( Bl Abf43) and applied it, together with GH10 and GH11 xylanases, to produce xylooligosaccharides (XOS) from wheat arabinoxylan and alkali pretreated sugarcane bagasse. The enzyme synergistically enhanced XOS production by GH10 and GH11 xylanases, being particularly efficient in combination with the latter family of enzymes, with a degree of synergism of 1.7. We also demonstrated that the enzyme is capable of not only removing arabinose decorations from the arabinoxylan and from the non -reducing end of the oligomeric substrates, but also hydrolyzing the xylan backbone yielding mostly xylobiose and xylose in particular cases. Structural studies of Bl Abf43 shed light on the molecular basis of the substrate recognition and allowed hypothesizing on the structural reasons of its multifunctionality.
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Univ Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Campbell, G. M.
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Mustac, N. Cukelj
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Univ Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Univ Zagreb, Fac Food Technol & Biotechnol, Zagreb, CroatiaUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Mustac, N. Cukelj
;
Alyassin, M.
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Univ Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Alyassin, M.
;
Gomez, L. D.
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Univ York, Dept Biol, Ctr Novel Agr Prod, York, N Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Gomez, L. D.
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Simister, R.
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Univ York, Dept Biol, Ctr Novel Agr Prod, York, N Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Simister, R.
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Flint, J.
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Univ Lincoln, Joseph Banks Labs, Coll Sci, Lincoln, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Flint, J.
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Philips, D. J.
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Biorenewables Dev Ctr, 1 Hassacarr Close,Chessingham Pk, York, N Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Philips, D. J.
;
Gronnow, M. J.
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Biorenewables Dev Ctr, 1 Hassacarr Close,Chessingham Pk, York, N Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Gronnow, M. J.
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Westwood, N. J.
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Univ St Andrews, Sch Chem & Biomed Sci, St Andrews, Fife, ScotlandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
机构:
Inst Nacl Tecnol Agr INTA, CICVyA, Inst Biotecnol, Los Reseros & N Repetto, RA-1686 Hurlingham, Buenos Aires, Argentina
Consejo Nacl Invest Cient & Tecn CONICET, Ciudad Autonoma, Buenos Aires, ArgentinaUniv Sao Paulo, Inst Fis Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP, Brazil
Campos, Eleonora
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Filgueiras, Jefferson Gonsalves
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Univ Fed Fluminense, Inst Quim, Outeiro Sao Joao Batista, BR-24020007 Niteroi, RJ, Brazil
Univ Fed Rio De Janeiro, Inst Fis, CP68528, BR-21941972 Rio De Janeiro, RJ, BrazilUniv Sao Paulo, Inst Fis Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP, Brazil
机构:
Univ Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Campbell, G. M.
;
Mustac, N. Cukelj
论文数: 0引用数: 0
h-index: 0
机构:
Univ Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Univ Zagreb, Fac Food Technol & Biotechnol, Zagreb, CroatiaUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Mustac, N. Cukelj
;
Alyassin, M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Alyassin, M.
;
Gomez, L. D.
论文数: 0引用数: 0
h-index: 0
机构:
Univ York, Dept Biol, Ctr Novel Agr Prod, York, N Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Gomez, L. D.
;
Simister, R.
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h-index: 0
机构:
Univ York, Dept Biol, Ctr Novel Agr Prod, York, N Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Simister, R.
;
Flint, J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Lincoln, Joseph Banks Labs, Coll Sci, Lincoln, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Flint, J.
;
Philips, D. J.
论文数: 0引用数: 0
h-index: 0
机构:
Biorenewables Dev Ctr, 1 Hassacarr Close,Chessingham Pk, York, N Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Philips, D. J.
;
Gronnow, M. J.
论文数: 0引用数: 0
h-index: 0
机构:
Biorenewables Dev Ctr, 1 Hassacarr Close,Chessingham Pk, York, N Yorkshire, EnglandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
Gronnow, M. J.
;
Westwood, N. J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ St Andrews, Sch Chem & Biomed Sci, St Andrews, Fife, ScotlandUniv Huddersfield, Sch Appl Sci, Huddersfield, W Yorkshire, England
机构:
Inst Nacl Tecnol Agr INTA, CICVyA, Inst Biotecnol, Los Reseros & N Repetto, RA-1686 Hurlingham, Buenos Aires, Argentina
Consejo Nacl Invest Cient & Tecn CONICET, Ciudad Autonoma, Buenos Aires, ArgentinaUniv Sao Paulo, Inst Fis Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP, Brazil
Campos, Eleonora
;
Filgueiras, Jefferson Gonsalves
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Fluminense, Inst Quim, Outeiro Sao Joao Batista, BR-24020007 Niteroi, RJ, Brazil
Univ Fed Rio De Janeiro, Inst Fis, CP68528, BR-21941972 Rio De Janeiro, RJ, BrazilUniv Sao Paulo, Inst Fis Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP, Brazil