Surface biomimetic modification with laminin-loaded heparin/poly-L-lysine nanoparticles for improving the biocompatibility

被引:31
作者
Liu, Tao [1 ]
Hu, Youdong [2 ]
Tan, Jianying [3 ]
Liu, Shihui [1 ]
Chen, Junying [3 ]
Guo, Xin [1 ]
Pan, Changjiang [1 ]
Li, Xia [2 ]
机构
[1] Huaiyin Inst Technol, Jiangsu Prov Key Lab Intervent Med Devices, Huaian, Peoples R China
[2] Xuzhou Med Coll, Affiliated Huaian Hosp, Dept Geriatr, Huaian, Peoples R China
[3] Southwest Jiaotong Univ, Sch Mat Sci & Engn, Chinese Educ Minist, Key Lab Adv Technol Mat, Chengdu, Peoples R China
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2017年 / 71卷
基金
中国国家自然科学基金;
关键词
Heparin; Biomimetic; Nanoparticles; Endothelialization; Hemocompatibility; TISSUE FACTOR EXPRESSION; VASCULAR CELLS BEHAVIOR; TITANIUM SURFACE; ENDOTHELIAL-CELL; ELUTING STENTS; KAPPA-B; ACTIVATION; DIRECTION; GROWTH; HEMOCOMPATIBILITY;
D O I
10.1016/j.msec.2016.11.010
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Late thrombus and restenosis caused by delayed endothelialization and insufficient biocompatibility of polymer coating continue to be the greatest limitations of drug-eluting stents. In this study, based on the specific structure of vascular basement membrane, a novel biomimetic nano-coating was constructed by incorporating laminin into electrostatic-assembled heparin/poly-L-lysine nanoparticles. Alteration of heparin and poly-L-lysine concentration ratio in a certain range has no significantly influence nanoparticle size, uniformity and stability, but may affect the chemical property and subsequently the binding efficiency to dopamine-coated titanium surface. By use of this feature, four different nanoparticles were synthesized and immobilized on titanium surface for creating gradient nanoparticle binding density. According to in vitro biocompatibility evaluation, the nanoparticle modified surfaces were found to effectively block coagulation pathway and reduce thrombosis formation, Moreover, NP10L and NP15L modified surface with relatively low heparin exposing density (4.9 to 7.1 mu g/cm(2)) showed beneficial effect in selective promoting EPCs and ECs proliferation, as well as stimulating cell migration and NO synthesis. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:929 / 936
页数:8
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