The Kinetic parameters, Thermodynamic parameters, and Mechanism of PLpro from SARS-CoV and SARS-CoV-2

被引：0

作者：

Kang, Da ^{[1
]}

Mei, Kunrong ^{[1
]}

Zhang, Jianyu ^{[1
]}

机构：

[1] Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin, Peoples R China

来源：

PROCEEDINGS OF 2023 4TH INTERNATIONAL SYMPOSIUM ON ARTIFICIAL INTELLIGENCE FOR MEDICINE SCIENCE, ISAIMS 2023 | 2023年

关键词：

PLpro; kinetic parameter; thermodynamic parameter; mechanism; PAPAIN-LIKE PROTEASE; INHIBITORS; RECOGNITION; POTENT;

D O I：

10.1145/3644116.3644322

中图分类号：

TP18 [人工智能理论];

学科分类号：

081104 ; 0812 ; 0835 ; 1405 ;

摘要：

The pandemic of Covid-19 has caused great harm to the world. Curbing the spread of the virus is therefore important to prevent and stop possible future outbreaks. The SARS-CoV-2 genome encodes an important papain-like protease (PLpro), which is located in its non-structural protein 3 (NSP3). PLpro is an essential cysteine protease, capable of cutting viral polyproteins, and has the ability to remove ubiquitin and ubiquitin-like proteins, so it is an important antiviral target. The kinetic and thermodynamic parameters of PLpro are essential to understanding the mechanism of enzymatic activity and inhibition. In this paper, the PLpro of SARS-CoV and SARS-CoV-2 are compared and introduced in detail, and the data of their kinetic parameters and thermodynamic parameters are reviewed. Finally, we explain the mechanism of PLpro function and the inhibitory mechanism of GRL0617.

引用

页码：1218 / 1222

页数：5

共 50 条

[21] Protein structural heterogeneity: A hypothesis for the basis of proteolytic recognition by the main protease of SARS-CoV and SARS-CoV-2 [J].

Behnam, Mira A. M. .

BIOCHIMIE, 2021, 182 :177-184

[22] Chalcone-amide, a privileged backbone for the design and development of selective SARS-CoV/SARS-CoV-2 inhibitors [J].

Valipour, Mehdi .

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2022, 240

[23] Seeing the T cell Immunity of SARS-CoV-2 and SARS-CoV: Believing the Epitope-Oriented Vaccines [J].

Yue, Can ;

Wang, Pengyan ;

Tian, Jinmin ;

Gao, George F. ;

Liu, Kefang ;

Liu, William J. .

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2023, 19 (13) :4052-4060

[24] Chemical Space Profiling of SARS-CoV-2 PLpro Using DNA-Encoded Focused Libraries [J].

Wang, Xudong ;

Zhu, Ying ;

Zhao, Qingyi ;

Lu, Weiwei ;

Xu, Yechun ;

Hu, Hangchen ;

Lu, Xiaojie .

ACS MEDICINAL CHEMISTRY LETTERS, 2024, 15 (04) :555-564

[25] Comprehensive Structural and Molecular Comparison of Spike Proteins of SARS-CoV-2, SARS-CoV and MERS-CoV, and Their Interactions with ACE2 [J].

Hatmal, Ma'mon M. ;

Alshaer, Walhan ;

Al-Hatamleh, Mohammad A., I ;

Hatmal, Malik ;

Smadi, Othman ;

Taha, Mutasem O. ;

Oweida, Ayman J. ;

Boer, Jennifer C. ;

Mohamud, Rohimah ;

Plebanski, Magdalena .

CELLS, 2020, 9 (12)

[26] Insights Into Drug Repurposing, as Well as Specificity and Compound Properties of Piperidine-Based SARS-CoV-2 PLpro Inhibitors [J].

Calleja, Dale J. ;

Kuchel, Nathan ;

Lu, Bernadine G. C. ;

Birkinshaw, Richard W. ;

Klemm, Theresa ;

Doerflinger, Marcel ;

Cooney, James P. ;

Mackiewicz, Liana ;

Au, Amanda E. ;

Yap, Yu Q. ;

Blackmore, Timothy R. ;

Katneni, Kasiram ;

Crighton, Elly ;

Newman, Janet ;

Jarman, Kate E. ;

Call, Melissa J. ;

Lechtenberg, Bernhard C. ;

Czabotar, Peter E. ;

Pellegrini, Marc ;

Charman, Susan A. ;

Lowes, Kym N. ;

Mitchell, Jeffrey P. ;

Nachbur, Ueli ;

Lessene, Guillaume ;

Komander, David .

FRONTIERS IN CHEMISTRY, 2022, 10

[27] 6-Thioguanine blocks SARS-CoV-2 replication by inhibition of PLpro [J].

Swaim, Caleb D. ;

Dwivedi, Varun ;

Perng, Yi-Chieh ;

Zhao, Xu ;

Canadeo, Larissa A. ;

Harastani, Houda H. ;

Darling, Tamarand L. ;

Boon, Adrianus C. M. ;

Lenschow, Deborah J. ;

Kulkarni, Viraj ;

Huibregtse, Jon M. .

ISCIENCE, 2021, 24 (09)

[28] Allosteric Regulation of 3CL Protease of SARS-CoV-2 and SARS-CoV Observed in the Crystal Structure Ensemble [J].

Kidera, Akinori ;

Moritsugu, Kei ;

Ekimoto, Toru ;

Ikeguchi, Mitsunori .

JOURNAL OF MOLECULAR BIOLOGY, 2021, 433 (24)

[29] Identification of Triazolopyrimidinyl Scaffold SARS-CoV-2 Papain-Like Protease (PLpro) Inhibitor [J].

Kralj, Sebastjan ;

Jukic, Marko ;

Bahun, Miha ;

Kranjc, Luka ;

Kolaric, Anja ;

Hodoscek, Milan ;

Ulrih, Natasa Poklar ;

Bren, Urban .

PHARMACEUTICS, 2024, 16 (02)

[30] Regulation of N-degron recognin-mediated autophagy by the SARS-CoV-2 PLpro ubiquitin deconjugase [J].

Ayala-Torres, Carlos ;

Liu, Jiangnan ;

Dantuma, Nico P. ;

Masucci, Maria G. .

AUTOPHAGY, 2025, 21 (05) :1019-1038

← 1 2 3 4 5 →