Platelet factor 4 promotes deep venous thrombosis by regulating the formation of neutrophil extracellular traps

被引：4
作者：
Li, Wenqiang [1 ,2 ]
Chi, Decai [3 ]
Ju, Shuai [1 ,2 ]
Zhao, Xinyi [4 ]
Li, Xiaoyan [1 ,2 ]
Zhao, Junjie [5 ]
Xie, Huiqi [4 ]
Li, Yao [1 ,2 ]
Jin, Jiaqi [6 ]
Mang, Ge [4 ]
Dong, Zhihui [1 ,2 ,7 ]
机构：
[1] Fudan Univ, Dept Vasc, Jinshan Hosp, Shanghai, Peoples R China
[2] Fudan Univ, Wound Ctr, Jinshan Hosp, Shanghai, Peoples R China
[3] Harbin Med Univ, Dept Vasc Surg, Affiliated Hosp 2, Harbin, Peoples R China
[4] Harbin Med Univ, Dept Cardiol, Affiliated Hosp 2, Harbin, Peoples R China
[5] Fourth Hosp Changsha City, Dept Gen Surg, Changsha, Peoples R China
[6] Harbin Med Univ, Dept Neurosurg, Affiliated Hosp 2, Harbin, Peoples R China
[7] Fudan Univ, Dept Vasc Surg, Zhongshan Hosp, Shanghai, Peoples R China
来源：
THROMBOSIS RESEARCH | 2024年 / 237卷
关键词：
Neutrophil extracellular traps; Platelet; Thrombosis; Deep venous thrombosis; VEIN THROMBOSIS; CHROMATIN DECONDENSATION; NET FORMATION; TISSUE FACTOR; MOUSE MODEL; THROMBOEMBOLISM; INFLAMMATION; PROPHYLAXIS; INCREASES; PAD4;
D O I：
10.1016/j.thromres.2024.03.005
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The presence of neutrophil extracellular traps (NETs) in thrombotic diseases has been extensively studied. The exact mechanism of NET formation in deep venous thrombosis (DVT) has not been largely studied. This study is aimed to explore the role of NETs and their interaction with platelet factor 4 (PF4) in DVT. In plasma samples from 51 healthy volunteers and 52 DVT patients, NET markers and PF4 were measured using enzyme-linked immunosorbent assays (ELISA). NET generation in blood samples from healthy subjects and DVT patients was analyzed by confocal microscopy and flow cytometry. The plasma levels of NETs were significantly elevated in DVT patients, and neutrophils from patients showed a stronger ability to generate NETs after treatment. PF4 was upregulated in plasma samples from DVT patients and mediated NET formation. NETs enhanced procoagulant (PCA) via tissue factor and activating platelets to induce procoagulant activity. In addition, we established an inferior vena cava ligation (IVC) model to examine the role of NETs in thrombogenicity in DVT. In conclusion, NET formation was mediated by PF4 and enhance the procoagulant activity in DVT.
引用
收藏
页码：52 / 63
页数：12
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