Protein from tiger nut meal extracted by deep eutectic solvent and alkali-soluble acid precipitation: A comparative study on structure, function, and nutrition

被引:10
作者
Jiang, Xinyu [1 ]
Gao, Feng [1 ]
Ma, Yongliang [1 ]
Huo, Ning [1 ]
Guo, Yujie [1 ]
Yu, Yali [1 ]
机构
[1] Jilin Univ, Coll Food Sci & Engn, 5333 Xian Rd, Changchun 130062, Peoples R China
关键词
Protein from tiger nut meal; Deep eutectic solvent; Alkali-soluble acid precipitation; Structure; Functional properties; Nutritional properties; OIL; PH;
D O I
10.1016/j.foodchem.2024.139608
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Protein from tiger nut meal (TNP) performance high nutritional value. This study optimized the extraction parameters for TNP (DES-TNP) using deep eutectic solvent, with HBD: HBA = 5:1, Liquid: Solid = 11:1, and the moisture content was 15 %. A comprehensive comparison was conducted with the protein extracted using alkalisoluble acid precipitation (ASAE-TNP). DES-TNP demonstrated significantly higher purity (76.21 +/- 2.59 %) than ASAE-TNP (67.48 +/- 1.11 %). Density functional theory confirmed the successful synthesis of DES and its strong interaction with TNP. Moreover, DES-TNP and ASAE-TNP were different in structure (microscopic, secondary, and tertiary) and molecular weight distribution. The discrepancy contributed to the different functional properties, DES-TNP exhibiting better solubility, emulsification and foaming properties at pH13 compared to ASAETNP. For nutritional properties, DES-TNP and ASAE-TNP exhibited similar amino acid composition and digestibility, but the total amino acid content of DES-TNP was higher. This study presented a novel method for the extraction and comprehensive utilization of TNP.
引用
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页数:11
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