Experimental Study on Rats with Critical-Size Bone Defects Comparing Effects of Autologous Bone Graft, Equine Bone Substitute Bio-Gen® Alone or in Association with Platelet-Rich Fibrin (PRF)

被引:2
作者
Ciobanu, Petru [1 ]
Danciu, Mihai [2 ]
Pascu, Andrei [2 ]
Gardikiotis, Ioannis [3 ]
Forna, Norin [1 ]
Sirbu, Mihnea Theodor [4 ]
Calistru, Anca-Elena [5 ]
Puha, Bogdan [1 ]
Veliceasa, Bogdan [1 ]
Sirbu, Paul-Dan [1 ]
机构
[1] Grigore T Popa Univ Med & Pharm Iasi, Dept Surg Orthoped & Traumatol 2, Iasi 700454, Romania
[2] Grigore T Popa Univ Med & Pharm Iasi, Dept Morphofunct Sci Morphopathol 1, Iasi 700454, Romania
[3] Grigore T Popa Univ Med & Pharm Iasi, Adv Ctr Res & Dev Expt Med, Iasi 700454, Romania
[4] Grigore T Popa Univ Med & Pharm Iasi, Fac Med, Iasi 700454, Romania
[5] Iasi Univ Life Sci, Res Inst Agr & Environm, 9 Sadoveanu Alley, Iasi 700490, Romania
关键词
autologous bone graft; critical-size bone defect; platelet-rich plasma; Bio-Gen (R); mu CT; histomorphometry; TIBIA; STRENGTH; REPAIR;
D O I
10.3390/polym16111502
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Background: A critical-sized bone defect (CsBD) is considered one that will not heal spontaneously and requires reconstruction. This study aims to compare the results of using different bone reconstructive techniques and to study the potential of platelet-rich fibrin (PRF) to enhance the healing properties of a bone substitute (BS). Methods: In this experimental study on rats, the treatment of critical-sized bone defects was carried out by analysing four groups: a control group in which the bone defect was left empty; a group treated with Bio-Gen (R); another group in which the defect was treated with PRF in combination with Bio-Gen (R); and the last that was treated with autologous bone graft (ABG). The defects were evaluated by microcomputed tomography (mu CT) and then histomorphometrically. Results: From both the histological and imagistic point of view, the best results were registered in the ABG group, followed by the group treated with Bio-Gen (R) with PRF, Bio-Gen (R) group, and control group, with statistically significant differences. Conclusions: A 5 mm defect in the rat radius can be considered critical. ABG showed the best results in treating the bone defect. PRF significantly enhanced the efficacy of Bio-Gen (R).
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页数:18
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