Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

被引:0
|
作者
Xu, Fadi [1 ]
Sun, Dongming [1 ]
Wang, Zhaojiang [1 ]
Li, Menglei [1 ]
Yin, Xiaolong [1 ]
Li, Hongxing [1 ]
Xu, Lili [1 ]
Zhao, Jianzhi [1 ]
Bao, Xiaoming [1 ]
机构
[1] Qilu Univ Technol, Shandong Acad Sci, Sch Bioengn, Key Lab Biobased Mat & Green Papermaking, 3501 Daxue Rd, Jinan 250353, Peoples R China
关键词
poplar; pretreatment; enzymolysis; cellulosic ethanol; C5/C6 co-fermentation Saccharomyces cerevisiae; LOADING ENZYMATIC-HYDROLYSIS; SODIUM-CHLORITE PRETREATMENT; LIGNOCELLULOSIC BIOMASS; SUGARCANE BAGASSE; ACID PRETREATMENT; SACCHARIFICATION; STILLAGE; PROGRESS; LIGNIN;
D O I
10.3390/microorganisms12061174
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Cellulosic ethanol is the key technology to alleviate the pressure of energy supply and climate change. However, the ethanol production process, which is close to industrial production and has a high saccharification rate and ethanol yield, still needs to be developed. This study demonstrates the effective conversion of poplar wood waste into fuel-grade ethanol. By employing a two-step pretreatment using sodium chlorite (SC)-dilute sulfuric acid (DSA), the raw material achieved a sugar conversion rate exceeding 85% of the theoretical value. Under optimized conditions, brewing yeast co-utilizing C6/C5 enabled a yield of 35 g/L ethanol from 10% solid loading delignified poplar hydrolysate. We increased the solid loading to enhance the final ethanol concentration and optimized both the hydrolysis and fermentation stages. With 20% solid loading delignified poplar hydrolysate, the final ethanol concentration reached 60 g/L, a 71.4% increase from the 10% solid loading. Our work incorporates the pretreatment, enzymatic hydrolysis, and fermentation stages to establish a simple, crude poplar waste fuel ethanol process, expanding the range of feedstocks for second-generation fuel ethanol production.
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页数:17
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