Label-Free Assessment of Neuron-Specific Enolase via Polydopamine over a Carbon-Nanotube-Based Flexible Immunosensor

被引：2

作者：

Mehta, Daisy ^{[1
]}

Thakur, Neha ^{[1
]}

Nagaiah, Tharamani C. ^{[1
]}

机构：

[1] Indian Inst Technol Ropar, Dept Chem, Rupnagar 140001, Punjab, India

来源：

ACS APPLIED BIO MATERIALS | 2024年

关键词：

NSE; immunosensor; ePDA/OCNT; AFM; SECM; screen-printed electrode; flexible immunosensor; FILMS; NANOPARTICLES; IMMUNOASSAY; MARKER;

D O I：

10.1021/acsabm.4c00514

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A label-free electrochemical immunosensor was developed for the rapid and sensitive detection of neuron-specific enolase (NSE). The electropolymerization of dopamine in conjunction with highly conductive carbon nanotubes offers a simple and quick platform for the direct anchoring of antibodies without the assistance of any coupling agent as well as a blocking agent. The developed immunosensor exhibited a wider detection range from 120 pM (9 ng mL(-1)) to 3 nM (200 ng mL(-1)) for NSE with a high sensitivity of 3.9 mu A pM(-1) cm(-2) in 0.1 M phosphate-buffered saline (PBS) at physiological pH (7.4). Moreover, the short recognition time (15 min) for the antigen enabled the detection to be fast and less invasive. Additionally, the evaluation of a rate constant at various concentrations of NSE via feedback mode of scanning electrochemical microscopy (SECM) explained the profound effect of antigen concentration on the rate of flow of electrons. Therefore, the proposed immunosensor can be a promising tool for the early detection of small cell lung cancer in a very short period of time with consistent accuracy.

引用

页码：4702 / 4709

页数：8