Melatonin Enhances Neural Differentiation of Adipose-Derived Mesenchymal Stem Cells

被引:6
作者
Romano, Ivana Roberta [1 ]
D'Angeli, Floriana [2 ]
Gili, Elisa [3 ]
Fruciano, Mary [3 ]
Lombardo, Giuseppe Angelo Giovanni [4 ,5 ]
Mannino, Giuliana [6 ]
Vicario, Nunzio [1 ]
Russo, Cristina [1 ]
Parenti, Rosalba [1 ]
Vancheri, Carlo [3 ]
Giuffrida, Rosario [1 ]
Pellitteri, Rosalia [7 ]
Lo Furno, Debora [1 ]
机构
[1] Univ Catania, Dept Biomed & Biotechnol Sci, I-95123 Catania, Italy
[2] San Raffaele Roma Open Univ, Dept Human Sci & Qual Life Promot, I-00166 Rome, Italy
[3] Univ Catania, Dept Clin & Expt Med, I-95124 Catania, Italy
[4] Univ Enna Kore, Fac Med & Surg, I-94100 Enna, Italy
[5] Cannizzaro Hosp, I-95126 Catania, Italy
[6] Univ Messina, Dept Chem Biol Pharmaceut & Environm Sci, I-98122 Messina, Italy
[7] CNR, Inst Biomed Res & Innovat, I-95126 Catania, Italy
关键词
adipose-derived mesenchymal stem cells; melatonin; glial conditioned media; neuronal differentiation; olfactory ensheathing cells; Schwann cells; melatonin receptors; SCHWANN-CELLS; NEUROGENIC DIFFERENTIATION; EXPRESSION; TRANSPLANTATION; PROLIFERATION; RECEPTORS; SIGNALS; GROWTH;
D O I
10.3390/ijms25094891
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Adipose-derived mesenchymal stem cells (ASCs) are adult multipotent stem cells, able to differentiate toward neural elements other than cells of mesodermal lineage. The aim of this research was to test ASC neural differentiation using melatonin combined with conditioned media (CM) from glial cells. Isolated from the lipoaspirate of healthy donors, ASCs were expanded in a basal growth medium before undergoing neural differentiation procedures. For this purpose, CM obtained from olfactory ensheathing cells and from Schwann cells were used. In some samples, 1 mu M of melatonin was added. After 1 and 7 days of culture, cells were studied using immunocytochemistry and flow cytometry to evaluate neural marker expression (Nestin, MAP2, Synapsin I, GFAP) under different conditions. The results confirmed that a successful neural differentiation was achieved by glial CM, whereas the addition of melatonin alone did not induce appreciable changes. When melatonin was combined with CM, ASC neural differentiation was enhanced, as demonstrated by a further improvement of neuronal marker expression, whereas glial differentiation was attenuated. A dynamic modulation was also observed, testing the expression of melatonin receptors. In conclusion, our data suggest that melatonin's neurogenic differentiation ability can be usefully exploited to obtain neuronal-like differentiated ASCs for potential therapeutic strategies.
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页数:20
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