3D-bioprinted alginate-based bioink scaffolds with β-tricalcium phosphate for bone regeneration applications

被引：11
作者：
Wu, Yi-Fan [1 ,2 ]
Wen, Ya-Ting [3 ]
Salamanca, Eisner [1 ]
Aung, Lwin Moe [1 ]
Chao, Yan-Qiao [1 ]
Chen, Chih-Yun [4 ]
Sun, Ying-Sui [5 ]
Chang, Wei-Jen [1 ,6 ]
机构：
[1] Taipei Med Univ, Coll Oral Med, Sch Dent, 250 Wu Hsing St, Taipei 11031, Taiwan
[2] Ming Chuan Univ, Dept Biomed Engn, Taoyuan, Taiwan
[3] Taichung Vet Gen Hosp, Dept Med Educ, Taichung, Taiwan
[4] Taipei Med Univ, Coll Oral Med, Sch Oral Hyg, Taipei, Taiwan
[5] Taipei Med Univ, Coll Oral Med, Sch Dent Technol, 250 Wu Hsing St, Taipei 11031, Taiwan
[6] Taipei Med Univ, Shuang Ho Hosp, Dent Dept, New Taipei, Taiwan
来源：
JOURNAL OF DENTAL SCIENCES | 2024年 / 19卷 / 02期
关键词：
Alginate; Beta-tricalcium phosphate ( beta-TCP); Bioprinting; Bone regeneration; MESENCHYMAL STEM-CELLS; OSTEOCHONDRAL ALLOGRAFTS; IN-VITRO; CARTILAGE; HYDROGELS; RESPONSES; MATRIX;
D O I：
10.1016/j.jds.2023.12.023
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Background/purpose: 3D-printed bone tissue engineering is becoming recognized as a key approach in dentistry for creating customized bone regeneration treatments fitting patients bone defects requirements. 3D bioprinting offers an innovative method to fabricate detailed 3D structures, closely emulating the native bone micro-environment and better bone regeneration. This study aimed to develop an 3D-bioprintable scaffold using a combination of alginate and beta-tricalcium phosphate (beta-TCP) with the Cellink (R) BioX printer, aiming to advance the field of tissue engineering. Materials and methods: The physical and biological properties of the resulting 3D-printed scaffolds were evaluated at 10 %, 12 %, and 15 % alginate combined with 10 % beta-TCP. The scaffolds were characterized through printability, swelling behavior, degradability, and element analysis. The biological assessment included cell viability, alkaline phosphatase (ALP) activity. Results: 10 % alginate/beta-TCP 3D printed at 25 degrees C scaffold demonstrated the optimal condition for printability, swelling capability, and degradability of cell growth and nutrient diffusion. Addition of beta-TCP particles significantly improved the 3D printed material viscosity over only alginate (P < 0.05). 10 % alginate/beta-TCP enhanced MG-63 cell's proliferation (P < 0.05) and alkaline phosphatase activity (P < 0.001). Conclusion: This study demonstrated in vitro that 10 % alginate/beta-TCP bioink characteristic for fabricating 3D acellular bioprinted scaffolds was the best approach. 10 % alginate/beta-TCP bioink 3D-printed scaffold exhibited superior physical properties and promoted enhanced cell viability and alkaline phosphatase activity, showing great potential for personalized bone regeneration treatments. (c) 2024 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
收藏
页码：1116 / 1125
页数：10
相关论文
共 57 条
[1]   Bioinks and bioprinting technologies to make heterogeneous and biomimetic tissue constructs [J].
Ashammakhi, N. ;
Ahadian, S. ;
Xu, C. ;
Montazerian, H. ;
Ko, H. ;
Nasiri, R. ;
Barros, N. ;
Khademhosseini, A. .
MATERIALS TODAY BIO, 2019, 1
[2]  
Avila Hector Martinez, 2016, Bioprinting, V1-2, P22, DOI 10.1016/j.bprint.2016.08.003
[3]   β-tricalcium phosphate for bone substitution: Synthesis and properties [J].
Bohner, Marc ;
Santoni, Bastien Le Gars ;
Dobelin, Nicola .
ACTA BIOMATERIALIA, 2020, 113 :23-41
[4]   Recent advances in bone tissue engineering scaffolds [J].
Bose, Susmita ;
Roy, Mangal ;
Bandyopadhyay, Amit .
TRENDS IN BIOTECHNOLOGY, 2012, 30 (10) :546-554
[5]   Poly-ε-caprolactone/hydroxyapatite composites for bone regeneration:: in vitro characterization and human osteoblast response [J].
Causa, F ;
Netti, PA ;
Ambrosio, L ;
Ciapetti, G ;
Baldini, N ;
Pagani, S ;
Martini, D ;
Giunti, A .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2006, 76A (01) :151-162
[6]   Biomaterials / bioinks and extrusion bioprinting [J].
Chen, X. B. ;
Anvari-Yazdi, A. Fazel ;
Duan, X. ;
Zimmerling, A. ;
Gharraei, R. ;
Sharma, N. K. ;
Sweilem, S. ;
Ning, L. .
BIOACTIVE MATERIALS, 2023, 28 :511-536
[7]   3D Bioprinting of shear-thinning hybrid bioinks with excellent bioactivity derived from gellan/alginate and thixotropic magnesium phosphate-based gels [J].
Chen, You ;
Xiong, Xiong ;
Liu, Xin ;
Cui, Rongwei ;
Wang, Chen ;
Zhao, Guoru ;
Zhi, Wei ;
Lu, Mengjie ;
Duan, Ke ;
Weng, Jie ;
Qu, Shuxin ;
Ge, Jianhua .
JOURNAL OF MATERIALS CHEMISTRY B, 2020, 8 (25) :5500-5514
[8]   Polycaprolactone/Hydroxyapatite composite scaffolds: Preparation, characterization, and in vitro and in vivo biological responses of human primary bone cells [J].
Chuenjitkuntaworn, Boontharika ;
Inrung, Wipawan ;
Damrongsri, Damrong ;
Mekaapiruk, Kongkwan ;
Supaphol, Pitt ;
Pavasant, Prasit .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2010, 94A (01) :241-251
[9]  
Datta S, 2019, Alginatesrecent uses of this natural polymer
[10]   Engineering bioinks for 3D bioprinting [J].
Decante, Guy ;
Costa, Joao B. ;
Silva-Correia, Joana ;
Collins, Maurice N. ;
Reis, Rui L. ;
Oliveira, J. Miguel .
BIOFABRICATION, 2021, 13 (03)
123456