Genomic analysis and antibiotic resistance profiling of Bacillus amyloliquefaciens AUPP2: implication for biocontrol agent against the fruit rot of banana

A Plant Growth-Promoting Rhizobacteria (PGPR), Bacillus amyloliquefaciens AUPP2 (Bam2) has antiviral and antibiotic properties. With the smallest sequence of 468,810 bp, the Bam2 genome has an overall length of 3,864,629base pairs. Notably, Bam2 harbor only two out of seventeen DNA islands identified in DVI-F-3, a feature contrasting with the B. amyloliquefaciens type strain DSM7, which possess "contig L" that Bam2 lacks. In addition, nine antibiotic resistance genes have been identified by analysis of DNA samples from Bam2 and other strains as an indication of the underlying mechanism for antimicrobial resistance. Bam2 is distinguished from other strains in that it has a high number of rare genes. In addition, in this study, genetic and physiological similarities between Bacillus subtilis and B. amyloliquefaciens are examined. The biochemical test shows positive result that confirm the Bam 2 strain. The maximum % inhibition over control results about 81.78% over the other strains. Specific DNA islands such as Contigs B, D, I, and L50 were identified with DVI-F-3, notably lacking "contigs D and I" while only possessing "contigs B and L50," which are relatively diminutive in size.