Optical-resolution photoacoustic microelastography system for elasticity mapping: Phantom study and practical application

Elastography is a noninvasive technique for characterizing the mechanical properties of biological tissues. Conventional methods have limitations in resolution and sensitivity, hindering disease detection in clinical diagnostics. To address these issues, this study developed an optical-resolution photoacoustic microelastography (OR-PAME) system. Using an agar tissue phantom with varying agar concentrations and contrast agents, PAME evaluated elasticity distribution under compression in both lateral and axial dimensions. It indirectly measured elastic properties by correlating photoacoustic responses, temporal lags, and induced displacement. We also applied the system to the study of the distribution of elastic characteristics of the liver tissue after ablation, which confirmed the potential of OR-PAME in the study of elastic characteristics. Quantitative analysis showed greater lateral displacement in regions with reduced agar concentrations, indicating decreased stiffness. PAME also detected vertical displacement along the axial plane, validating its efficacy in elastographic imaging. By improving resolution and penetration, PAME provides superior visualization of elasticity distribution. Its methodology correlates microstructural alterations with tissue biomechanics, holding potential implications in medical diagnostics. In this study, we utilized an optical-resolution photoacoustic microscopy imaging system to conduct photoacoustic imaging on an agar phantom with elastic differences and liver tissue after microwave ablation. By performing cross-correlation calculations, we obtained the distribution of elastic characteristics. The results highlight the potential of the photoacoustic system in investigating the elastic properties of microwave-ablated tissues, presenting a novel approach for disease diagnosis and pathological tissue analysis. image