Elastin-Like Peptide as a Model for Disordered Proteins: Diffusion Behaviour in Self-Crowding Conditions

被引：2

作者：

Weissheit, Susann ^{[1
]}

Kuttich, Bjoern ^{[2
]}

Vogel, Michael ^{[2
]}

Thiele, Christina Marie ^{[1
]}

机构：

[1] Tech Univ Darmstadt, Clemens Schopf Inst Organ Chem & Biochem, Peter-Grunberg-Str 16, D-64287 Darmstadt, Germany

[2] Tech Univ Darmstadt, Inst Phys Kondensierter Materie, Hochsch str 6, D-64289 Darmstadt, Germany

来源：

CHEMPHYSCHEM | 2024年 / 25卷 / 13期

关键词：

intrinsically disordered proteins; elastin-like peptides; diffusion; crowding; NMR spectroscopy; GENERALIZED CONCENTRATION; TRANSLATIONAL DIFFUSION; DEUTERIUM-OXIDE; HEAVY-WATER; TEMPERATURE; TRANSITION; VISCOSITY; POLYMERS; DYNAMICS; RANGE;

D O I：

10.1002/cphc.202400117

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Despite the current high interest, there is limited information on diffusion data for intrinsically disordered proteins (IDPs). This study investigates the effect of crowding on the diffusion behaviour of an elastin-like peptide (ELP), by combined pulse field gradient (PFG) and static field gradient (SFG) NMR techniques. We interpret our findings in terms of highly dynamic chain assemblies with weak interactions, resulting in ELP diffusion that is primarily governed by the viscous flow of the solvent. The diffusion behaviour of the peptide appears to resemble that of globular proteins rather than flexible linear polymers over a wide concentration range.

引用

页数：9