Low Immunogenicity of Keratinocytes Derived from Human Embryonic Stem Cells

被引：0

作者：

Shen, Jiayi ^{[1
,2
]}

Zeng, Xuanhao ^{[1
,2
]}

Lv, Haozhen ^{[3
,4
]}

Jin, Yiting ^{[5
]}

Liu, Yating ^{[1
,2
]}

Lian, Weiling ^{[1
,2
]}

Huang, Shiyi ^{[1
,2
]}

Zang, Qing ^{[1
,2
]}

Zhang, Qi ^{[1
,2
]}

Xu, Jinhua ^{[1
,2
]}

机构：

[1] Fudan Univ, Huashan Hosp, Dept Dermatol, Shanghai 200040, Peoples R China

[2] Shanghai Inst Dermatol, Shanghai 200443, Peoples R China

[3] Beijing Hosp, Natl Ctr Gerontol, Dept Dermatol, Beijing 100730, Peoples R China

[4] Chinese Acad Med Sci, Inst Geriatr Med, Beijing 100730, Peoples R China

[5] Fudan Univ, Huashan Hosp, Dept Thyroid & Breast Surg, Gen Surg, Shanghai 200040, Peoples R China

来源：

CELLS | 2024年 / 13卷 / 17期

基金：

中国国家自然科学基金;

关键词：

keratinocytes; embryonic stem cells; differentiation; allograft rejection; immunogenicity; MHC CLASS-I; MOUSE MODELS; SKIN; EXPRESSION; PROTEINS;

D O I：

10.3390/cells13171447

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Epidermal transplantation is a common and widely used surgical technique in clinical medicine. Derivatives of embryonic stem cells have the potential to serve as a source of transplantable cells. However, allograft rejection is one of the main challenges. To investigate the immunogenicity of keratinocytes derived from human embryonic stem cells (ESKCs), we conducted a series of in vivo and in vitro experiments. The results showed that ESKCs have low HLA molecule expression, limited antigen presentation capabilities, and a weak ability to stimulate the proliferation and secretion of inflammatory factors in allogeneic PBMCs in vitro. In humanized immune mouse models, ESKCs elicited weak transplant rejection responses in the host. Overall, we found that ESKCs have low immunogenicity and may have potential applications in the field of regenerative medicine.

引用

页数：14

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