Enhanced uric acid detection using functionalized multi-walled carbon nanotube/AgNi nanocomposites: A comparative study on screen-printed carbon electrode (SPCE) and fabric-based biosensors

In the development of biosensors, it is essential to have sensors that provide rapid responses, exhibit high sensitivity and selectivity, and are non-invasive, such as screen-printed carbon electrode-based biosensors. In this study, SPCE-based and fabric-based biosensors were fabricated by modifying the working electrode (WE) surface using functionalized Multi-walled Carbon Nanotube/AgNi nanocomposites (f-MWCNT/AgNi) to enhance the biosensor's performance in detecting uric acid (UA). The successful synthesis of the f-MWCNT/AgNi nano- material was confirmed through UV-Vis, Raman, SEM-EDX, and XRD analyses. The f-MWCNT/AgNi nano- materials were deposited on the WE surface using drop-casting. Subsequently, electrochemical characteristic tests and UA detection performance were conducted using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. The DPV curves revealed sensitivities of 27.699 mu A/mM and 4.638 mu A/mM for SPCEbased and fabric-based electrodes, respectively. The limit of detection (LOD) values for UA detection were 0.024 and 0.017 mM, with linearity (R2) 2 ) 0.997 and 0.999 observed within the linear ranges of 0.05-1.00 and 1.0-5.0 mM, respectively. Both biosensors exhibited strong selectivity for UA against other components, including ascorbic acid, glucose, lactic acid, and ethanol. Based on these parameter values, f-MWCNT/AgNi-modified SPCE and fabric-based electrodes can be promoted as biosensors for uric acid detection.