Metabolomic changes following GenX and PFBS exposure in developing zebrafish

被引:3
作者
Dunn, Fiona [1 ]
Paquette, Shannon E. [2 ]
Pennell, Kurt D. [1 ]
Plavicki, Jessica S. [2 ]
Manz, Katherine E. [1 ,3 ]
机构
[1] Brown Univ, Sch Engn, 184 Hope St, Providence, RI 02912 USA
[2] Brown Univ, Dept Pathol & Lab Med, 70 Ship St, Providence, RI 02903 USA
[3] Univ Michigan, Dept Environm Hlth Sci, 1415 Washington Hts, Ann Arbor, MI 48109 USA
关键词
GenX; PFBS; Zebrafish; Embryonic development; Metabolomics; Body burden; PERFLUOROBUTANE SULFONATE PFBS; PERFLUOROOCTANE SULFONATE; PURINE METABOLISM; DANIO-RERIO; TOXICITY; DEREGULATION; SUBSTANCES; WATER; ACIDS; FISH;
D O I
10.1016/j.aquatox.2024.106908
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
Short chain per- and polyfluoroalkyl substances (PFAS), including hexafluoropropylene oxide dimer acid (GenX) and perfluorobutane sulfonate (PFBS), are replacement chemicals for environmentally persistent, long-chain PFAS. Although GenX and PFBS have been detected in surface and ground water worldwide, few studies provide information on the metabolic alterations or risks associated with their exposures. In this study, larval zebrafish were used to investigate the toxicity of early-life exposure to GenX or PFBS. Zebrafish were chronically exposed from 4 h post-fertilization (hpf) to 6 days post-fertilization (dpf) to 150 mu M GenX or 95.0 mu M PFBS. Ultrahigh-performance liquid chromatography paired with high-resolution mass spectrometry was used to quantify uptake of GenX and PFBS into zebrafish larvae and perform targeted and untargeted metabolomics. Our results indicate that PFBS was 20.4 % more readily absorbed into the zebrafish larvae compared to GenX. Additionally, PFBS exposure significantly altered 13 targeted metabolites and 21 metabolic pathways, while GenX exposure significantly altered 1 targeted metabolite and 17 metabolic pathways. Exposure to GenX, and to an even greater extent PFBS, resulted in a number of altered metabolic pathways in the amino acid metabolism, with other significant alterations in the carbohydrate, lipid, cofactors and vitamins, nucleotide, and xenobiotics metabolisms. Our results indicate that GenX and PFBS impact the zebrafish metabolome, with implications of global metabolic dysregulation, particularly in metabolic pathways relating to growth and development.
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页数:10
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