A non-peptide-based chymotrypsin-targeted long-wavelength emission fluorescent probe with large Stokes shift and its application in bioimaging

被引：3

作者：

Fan, Chuanfeng ^{[1
]}

Gao, Jian ^{[1
]}

Gao, Yingkai ^{[1
]}

Yang, Xintong ^{[1
]}

Li, Gaoning ^{[1
]}

Wang, Xiaochun ^{[1
,2
]}

Li, Fei ^{[3
]}

Zhou, Jin ^{[4
]}

Yu, Haifeng ^{[5
]}

Huang, Yi ^{[3
]}

Chen, Jin ^{[1
]}

Shan, Yingying ^{[1
]}

Chen, Li ^{[1
]}

机构：

[1] Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Key Lab Opt Elect Sensing & Analyt Chem Life Sci, MOE,Shandong Prov Key Lab Biochem Engn,Qingdao Nuc, Qingdao 266042, Peoples R China

[2] Anshan Normal Univ, Sch Chem & Life Sci, Liaoning Key Lab Dev & Utilizat Nat Prod Act Mol, Anshan 114007, Peoples R China

[3] Army Med Univ, Biomed Anal Ctr, Chongqing 400038, Peoples R China

[4] Weifang Med Univ, Sch Pharm, Weifang 261053, Peoples R China

[5] Baicheng Normal Univ, Coll Chem, Baicheng 137000, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2024年 / 35卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Chymotrypsin; Fluorescent probe; Long -wavelength emission; Large Stokes shift; Bioimaging; HEAT-STROKE; DESIGN; LEVEL; SERUM;

D O I：

10.1016/j.cclet.2024.109838

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

As a hydrolase, chymotrypsin (CHT) is involved in many physiological activities, and its abnormal activity is closely related to diabetes, pancreatic fibrosis, chronic pancreatitis and pancreatic cancer. In this work, an innovative long-wavelength emission fluorescent probe TCF-CHT was designed and synthesized for the high specificity detection of CHT, which utilized TCF-OH and a mimetic peptide substrate 4-bromobutyryl as chromogenic group and recognition group, respectively. TCF-CHT exhibited excellent selectivity and eye-catching sensitivity (8.91 ng/mL) towards CHT, "off-on" long-wavelength emission at 670 nm and large Stokes shift (140 nm). Furthermore, the successful fulfillment and perfect performance in imaging endogenous CHT in complex organisms (P815 cells, HepG2 cells, zebrafish and tumor-bearing mice) verified its potential as a powerful tool for the recognition of CHT in complicated biological environments. (c) 2024 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.

引用

页数：7

共 64 条

[51] A water-soluble fluorescent probe for the determination of ?-glutamyltransferase activity and its application in tumor imaging [J].