Value of Metagenomic Next-Generation Sequencing in Clinical Diagnosis and Targeted Therapy of Nontuberculous Mycobacteria Infection

被引：1

作者：

Xiao, Lanlan ^{[1
]}

Zhao, Shuai ^{[1
]}

Wang, Hongjuan ^{[2
]}

Yu, Liang ^{[1
]}

Guo, Jing ^{[1
]}

Wu, Xinyi ^{[3
]}

Xu, Xiaowei ^{[1
]}

机构：

[1] Zhejiang Univ, Affiliated Hosp 1, Collaborat Innovat Ctr Diag & Treatment Infect Dis, Sch Med,Natl Clin Res Ctr Infect Dis,Natl Med Ctr, 79 Qingchun Rd, Hangzhou 310003, Peoples R China

[2] Nantong Univ, Affiliated Hosp, Nantong, Peoples R China

[3] Zhejiang Univ City Coll, Hangzhou, Peoples R China

来源：

INFECTIOUS MICROBES & DISEASES | 2024年 / 6卷 / 02期

关键词：

nontuberculous mycobacteria; metagenomic next-generation sequencing; mycobacterial culture; turnaround time; LABORATORY DIAGNOSIS; TUBERCULOSIS; CULTURE; SPUTUM;

D O I：

10.1097/IM9.0000000000000135

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The purpose of this study was to assess the value of metagenomic next-generation sequencing (mNGS) for rapid diagnosis of diseases caused by nontuberculous mycobacteria (NTM). We retrospectively reviewed four NTM-infected cases diagnosed by smear microscopy, mycobacterial culture and mNGS methods. We found that the mNGS method not only had a shorter detection turnaround time (3-4 days) than Mycobacterium culture (15-20 days) but also had higher sensitivity and specificity to identify NTM compared with conventional detection methods. In addition, mNGS was able to identify coinfections by NTM and other bacteria, fungi or viruses. Overall, diagnosis of NTM by mNGS can provide timely and precise guidance for subsequent clinical treatment of NTM infections.

引用

页码：100 / 102

页数：3

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