Melatonin alleviates UV-B stress and enhances phenolic biosynthesis in rosemary (Rosmarinus officinalis) callus

被引：0

作者：

Zhao, Zhuowen ^{[1
,2
]}

Liu, Siyu ^{[1
]}

Yun, Cholil ^{[3
,4
]}

Liu, Jianing ^{[3
]}

Yao, Liuyang ^{[3
]}

Wang, Huimei ^{[1
]}

机构：

[1] Zhejiang A&F Univ, Coll Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou, Zhejiang, Peoples R China

[2] Cangzhou Tech Coll, Dept Agr & Anim Husb Engn, Cangzhou, Hebei, Peoples R China

[3] Northeast Forestry Univ, Coll Chem Chem Engn & Resource Utilizat, Key Lab Forest Plant Ecol, Minist Educ,Heilongjiang Prov Key Lab Ecol Utiliza, Harbin, Peoples R China

[4] Kim Il Sung Univ, Coll Forest Sci, Pyongyang, North Korea

来源：

PHYSIOLOGIA PLANTARUM | 2024年 / 176卷 / 04期

关键词：

NITRIC-OXIDE; SECONDARY METABOLITES; CARNOSIC ACID; ESSENTIAL OIL; ROOT-GROWTH; ANTIOXIDANT; PERFORMANCE; CULTURE; TOLERANCE; MOLECULES;

D O I：

10.1111/ppl.14453

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Although used in in vitro culture to boost secondary metabolite production, UV-B radiation can seriously affect plant growth if not properly dosed. Rosemary callus can be used as an important source of effective ingredients in the food and medicine industry. To balance the positive and negative effects of UV-B on rosmary callus, this study investigated the effects of melatonin on rosemary callus under UV-B radiation. The results showed that melatonin improved rosemary callus growth, with fresh weight and dry weight increased by 15.81% and 8.30%, respectively. The addition of 100 mu M melatonin increased antioxidant enzyme activity and NO content in rosemary callus. At the same time, melatonin also significantly reduced membrane lipid damage and H2O2 accumulation in rosemary callus under UV-B stress, with malondialdehyde (MDA) and H2O2 contents reduced by 13.03% and 14.55%, respectively. In addition, melatonin increased the total phenol and rosmarinic acid contents in rosemary callus by 19% and 54%, respectively. Melatonin significantly improved the antioxidant activity of the extracts from rosemary callus. These results suggest that exogenous melatonin can alleviate the adverse effects of UV-B stress on rosemary callus by promoting NO accumulation while further enhancing phenolic accumulation and biological activity.

引用

页数：10