PHYTOCHEMICAL ANALYSIS, ANTIOXIDANT ACTIVITY AND ANTIBACTERIAL ACTIVITY OF ILEX DIPYRENA

The utilization of botanical flora and plant-derived therapeutics is extensively prevalent within indigenous communities globally. Furthermore, in contemporary society, there is a growing inclination to embrace these natural sources as supplements or alternatives to synthetic pharmaceutical agents. This ongoing investigation is concerned with the comprehensive examination of phytochemical constituents, molecular attributes, and antibacterial efficacy inherent to the Ilex dipyrena plant. The investigation revealed that the plant's butanol fraction exhibited good antibacterial activity, particularly against E. faecalis, when compared to various other extracts and bacterial strains under scrutiny, presenting a zone of inhibition of 26.00 +/- 0.33 mm. The botanical specimen demonstrated substantial composition of flavonoids, saponins, carbohydrates, and tannins. Additionally, minute quantities of alkaloids, proteins, and lipids were discerned. Commendable antioxidant activity has been shown by the butanol fraction both in DPPH and ABTS methods. In DPPH assay, the butanol fraction has displayed the highest antioxidant activity as 91.36% inhibition while in ABTS test, the inhibition noted was 88.88% at 1000 mu g/ml with the IC(50 )value of 20.91 mu g/ml. Gas chromatography-mass spectrometry (GC-MS) analysis has revealed the presence of significant bioactive compounds within the samples. These compounds include coumaran, 2-Hexyl hydroperoxide, Proline, 5-0xo, methyl ester, 4-((1E)-3-Hydroxyl-1-propnyl)-2-methoxyphenol, Cinnamic acid p-hydroxy-methyl ester, Tetradecanoic acid, 1Octadecyne, n-Hexadecanoic acid, 3-(2,5-dimethoxyphenyl) propionic acid, Linoleic acid, methyl ester, Phytol, 9-12,15octadecatrienoic acid and Octadecanoic acid.